CAJ | 학술논문

以鱼粉为脂肪源,设脂肪水平分别为6.1%(A组)、9.5%(B组)、12.4%(C组)、15.1%(D组)、18.5%(E组)的5种实验饲料,对平均初始体质量为(27.56±0.19) g的褐菖(Sebastiscus marmoratus)进行为期60 d 的饲养实验,探鲉讨饲料脂肪水平对褐菖血清生化指标、免疫及抗氧化酶活力的影响.结果表明:(1)随着饲料中脂肪水平的升高,褐菖鲉血清中的高密度脂蛋白胆固醇(HDL-C)呈逐渐升高的趋势, E组最高,显著高于A组、B组和C组(P<0.05),低密度脂蛋白胆固醇(LDL-C)表现为先上升后下降的趋势, C组显著高于D组和E组(P<0.05),而甘油三酯(TG)和总胆固醇(CHO)呈下降的趋势, D组、E组显著低于A组、B组和C组(P<0.05),总蛋白(TP)含量在各组间差异不显著(P>0.05);(2)褐菖鲉血清免疫球蛋白M(IgM)含量随脂肪水平的升高呈现先上升后下降的趋势, C组显著高于其他各组(P<0.05),溶菌酶(LZM)活力也呈现先上升后下降的趋势, C组显著高于A组、D组和E组(P<0.05);(3)肝超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力和总抗氧化能力(T-AOC)随着饲料中脂肪水平的升高呈现先上升后下降的趋势, C组显著高于其他各组(P<0.05),丙二醛(MDA)含量则呈现逐渐上升的趋势,其D组和E组显著高于其他各组(P<0.05).血清中SOD活力随脂肪水平升高呈现上升趋势, D组和E组显著高于A组、B组、C组(P<0.05);血清 CAT 活力也表现为上升的趋势,但各组之间差异不显著(P>0.05).综上可见,饲料中适宜的脂肪水平(12.4%)鲉可明显改善褐菖的免疫功能与抗氧化能力.鲉
이어분위지방원,설지방수평분별위6.1%(A조)、9.5%(B조)、12.4%(C조)、15.1%(D조)、18.5%(E조)적5충실험사료,대평균초시체질량위(27.56±0.19) g적갈창(Sebastiscus marmoratus)진행위기60 d 적사양실험,탐유토사료지방수평대갈창혈청생화지표、면역급항양화매활력적영향.결과표명:(1)수착사료중지방수평적승고,갈창유혈청중적고밀도지단백담고순(HDL-C)정축점승고적추세, E조최고,현저고우A조、B조화C조(P<0.05),저밀도지단백담고순(LDL-C)표현위선상승후하강적추세, C조현저고우D조화E조(P<0.05),이감유삼지(TG)화총담고순(CHO)정하강적추세, D조、E조현저저우A조、B조화C조(P<0.05),총단백(TP)함량재각조간차이불현저(P>0.05);(2)갈창유혈청면역구단백M(IgM)함량수지방수평적승고정현선상승후하강적추세, C조현저고우기타각조(P<0.05),용균매(LZM)활력야정현선상승후하강적추세, C조현저고우A조、D조화E조(P<0.05);(3)간초양화물기화매(SOD)활력、과양화경매(CAT)활력화총항양화능력(T-AOC)수착사료중지방수평적승고정현선상승후하강적추세, C조현저고우기타각조(P<0.05),병이철(MDA)함량칙정현축점상승적추세,기D조화E조현저고우기타각조(P<0.05).혈청중SOD활력수지방수평승고정현상승추세, D조화E조현저고우A조、B조、C조(P<0.05);혈청 CAT 활력야표현위상승적추세,단각조지간차이불현저(P>0.05).종상가견,사료중괄의적지방수평(12.4%)유가명현개선갈창적면역공능여항양화능력.유
We evaluated the effects of dietary lipid levels on serum biochemistry indices, immunity, and antioxi-dant function in Sebastiscus marmoratus. Juvenile fish (average initial body weight:27.56±0.19 g) were randomly divided into 5 groups and fed diets containing different levels of lipid (from fish meal) for 60 d:diet A (6.1%), diet B (9.5%), diet C (12.4%), diet D (15.1%), or diet E (18.5). The HDL-C content tended to increase with increasing dietary lipid levels. HDL-C levels were highest in the fish fed diet E and were significantly higher than in fish fed diets A–C (P<0. 05). The LDL-C content also tended to increase at first but then decreased at higher dietary lipid levels. The LDL-C content was significantly higher in fish fed diet C than those fed diets D and E (P<0. 05). TG and CHO content tended to decrease as dietary lipid levels increased. TG and CHO content were significantly lower in fish fed diets D and E than in those fed diets A-C (P<0. 05). Diet had no effect on TP (P>0. 05). The IgM content tended to increase then decrease with increasing dietary lipid levels. IgM levels were significantly higher in fish fed diet C than all other groups. Similarly, LZM levels increased then decreased with increasing dietary lipid levels and were significantly higher in fish fed diet C than those fed diets A, D, and E. The liver SOD, CAT, and T-AOC activity also increased then decreased with increasing dietary lipid levels. Enzyme activity was highest in fish fed diet C than all other groups. The liver MDA content increased with increasing dietary lipid levels and was higher in fish fed diets D and E than those fed diets A?C (P<0. 05). Serum SOD activity also increased with increasing dietary lipid levels and was higher in fish fed diets D and E than those fed diets A?C (P<0. 05). Last, serum CAT activity tended to increase but there was no difference among the groups (P>0. 05). In conclusion, the adaptability of S. marmoratus to the dietary lipid levels is reflected by changes in serum biochemistry. Our data suggest the optimal dietary lipid levels (12.4%) are associated with improved immunity and antioxidant function.