CAJ | 학술논문

目的分析烧伤感染对血清中补体 C3和 IL-10水平的影响,旨在根据其变化水平及时给予合适的免疫调节治疗,预防感染的发生.方法分别采集患者烧伤后第3、7、14天的创面分泌物和外周血,对分泌物做细菌培养,用酶联免疫吸附测定法(Enzyme-linked immunosorbent assay, ELISA)检测血清中的补体 C3和 IL-10水平,病情严重疑有脓毒血症者及时进行血培养.结果烧伤后创面感染率不断上升,脓毒血症发生率达16.4% ,对补体 C3水平的影响无统计学意义(P >0.05),但烧伤后第14天脓毒血症患者血清中 IL-10水平是创面感染患者的2倍,相比有统计学意义(P <0.05).结论补体 C3水平升高有利于提高机体抵抗力,但不能反映机体的感染情况; IL-10水平过度升高对感染加重有一定的预警作用,在临床判断过程中辅助监测血清中 IL-10水平有助于发现感染加重的趋势,以便及时给予免疫调节治疗.
목적분석소상감염대혈청중보체 C3화 IL-10수평적영향,지재근거기변화수평급시급여합괄적면역조절치료,예방감염적발생.방법분별채집환자소상후제3、7、14천적창면분비물화외주혈,대분비물주세균배양,용매련면역흡부측정법(Enzyme-linked immunosorbent assay, ELISA)검측혈청중적보체 C3화 IL-10수평,병정엄중의유농독혈증자급시진행혈배양.결과소상후창면감염솔불단상승,농독혈증발생솔체16.4% ,대보체 C3수평적영향무통계학의의(P >0.05),단소상후제14천농독혈증환자혈청중 IL-10수평시창면감염환자적2배,상비유통계학의의(P <0.05).결론보체 C3수평승고유리우제고궤체저항력,단불능반영궤체적감염정황; IL-10수평과도승고대감염가중유일정적예경작용,재림상판단과정중보조감측혈청중 IL-10수평유조우발현감염가중적추세,이편급시급여면역조절치료.
Objective To analyze the influence of burn infection on complement C3 and IL-10 level in blood serum with the purpose of providing timely and proper immunoregulation based on the level changes and preventing the occurrence of infection. Methods Collect the secretion and the peripheral blood on the wounds respectively at the third, seventh and four-teenth day postburn. Do bacterial culture to the secretion and use ELISA method to test the levels of complement C3 and IL-10 level in blood serum. For serious cases that were suspected with septicopyemia, the blood culture was performed immediately. Results The postburn wound infection rate increased continuously and the incidence of septicopyemia also reached 16. 4% . But the effects they exerted on the level of complement C3 had no statistical significance (P > 0. 05). However, at the four-teenth day postburn, the level of IL-10 in blood serum of patients with septicopyemia was two times over that of the patients with burn infection, and the comparison had significance in statistics (P <0. 05). Conclusion The increase of complement C3 level can improve the body immunity but can not reflect the infection situation of the body, while the excessive increase of IL-10 level signifies the aggravation of infection to some extent, and in clinic, the auxiliary detection of IL-10 level in blood serum can help to discover the tendency of infection aggravation and so that prompt immunoregulation can be given.