CAJ | 학술논문

目的探讨烧伤湿性医疗技术(MEBT/ MEBO)及配套产品湿润烧伤膏(MEBO)对大鼠慢性难愈性皮肤溃疡创面肉芽组织表皮生长因子(EGF)、表皮细胞生长因子受体(EGFR)表达的影响.方法SPF 级SD 雄性大鼠45只,参照付小兵大鼠慢性性皮肤溃疡创面模型制作方法制作模型,成模后再采用随机数字表法随机分为模型组(15只)、 MEBO 组(15只)、贝复剂组(15只),造模后第2天开始用药,观察用药后各组创面愈合情况、肉芽组织生长情况,记录创面愈合时间;常规病理切片观察创面炎症、成纤维细胞数量、血管改变情况;透射电镜观察成纤维细胞及其线粒体形态结构;换药后第7、14天取创面组织,采用免疫组织化学法(SP法)观察 MEBO 对创面肉芽组织 EGF、 EGFR 表达的影响.结果MEBO 组大鼠的创面愈合情况明显好于模型组与贝复济组;创面愈合时间明显短于模型组及贝复济组(P <0.05; P <0.01);病理学观察: MEBO 组与贝复剂组及模型组比较,炎症反应轻、肉芽组织生长良好,纤维增生程度低;换药后第7、14天, MEBO 组大鼠创面肉芽组织中 EGF 和 EGFR 蛋白表达阳性染色指数分别较模型组和贝复济组大鼠创面肉芽组织中 EGF 和 EGFR 蛋白表达阳性染色指数提高,差异有统计学意义(P <0.05).结论MEBO 能增加慢性皮肤溃疡创面肉芽组织中EGF、 EGFR 的表达量,从而促进慢性皮肤溃疡愈合.
목적탐토소상습성의료기술(MEBT/ MEBO)급배투산품습윤소상고(MEBO)대대서만성난유성피부궤양창면육아조직표피생장인자(EGF)、표피세포생장인자수체(EGFR)표체적영향.방법SPF 급SD 웅성대서45지,삼조부소병대서만성성피부궤양창면모형제작방법제작모형,성모후재채용수궤수자표법수궤분위모형조(15지)、 MEBO 조(15지)、패복제조(15지),조모후제2천개시용약,관찰용약후각조창면유합정황、육아조직생장정황,기록창면유합시간;상규병리절편관찰창면염증、성섬유세포수량、혈관개변정황;투사전경관찰성섬유세포급기선립체형태결구;환약후제7、14천취창면조직,채용면역조직화학법(SP법)관찰 MEBO 대창면육아조직 EGF、 EGFR 표체적영향.결과MEBO 조대서적창면유합정황명현호우모형조여패복제조;창면유합시간명현단우모형조급패복제조(P <0.05; P <0.01);병이학관찰: MEBO 조여패복제조급모형조비교,염증반응경、육아조직생장량호,섬유증생정도저;환약후제7、14천, MEBO 조대서창면육아조직중 EGF 화 EGFR 단백표체양성염색지수분별교모형조화패복제조대서창면육아조직중 EGF 화 EGFR 단백표체양성염색지수제고,차이유통계학의의(P <0.05).결론MEBO 능증가만성피부궤양창면육아조직중EGF、 EGFR 적표체량,종이촉진만성피부궤양유합.
Objective To explore the effects of MEBT/ MEBO (i. e. Moist Exposed Burn Therapy and Moist Ex-posed Burn Ointment) on the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the wound granulation tissue of rat chronic skin ulcer. Methods Select 45 SPF SD male rats to make the rat chronic skin ulcerative wound models referring to the modeling method of FU Xiao-bin (Reference [1]). After the formation of the mod-el, randomly divide the 45 rats into three groups: model group (15 rats), MEBO group (15 rats) and rb-bFGF (Recombi-nant Bovine Basic Fibroblast Growth Factor for External Use) group (15 rats). From the second day after the model forma-tion, begin to apply the medications on the wounds. The wound healing situation and the growth situation of granulation were observed, and the wound healing time was recorded. Take routine pathological section to observe the inflammatory situation, amount of fibroblasts, changes of blood vessels. Use transmission electron microscope to observe the morphology and struc-ture of fibroblasts and their mitochondria. At the seventh and fourteenth days of dressing change, take the wound tissue and observe the effect of MEBO on the expression of EGF and EGFR in granulation tissue by using the Immunohistochemical method (SP method). Results The healing situation and the healing time in MEBO group were both superior to that in the other two groups (P < 0. 05, P < 0. 01). Pathological observation results: compared with the other two groups, MEBO group showed slighter inflammation, much better growth of granulation tissue and slighter fibroplasia. At the seventh and fourteenth days of dressing change, the positive staining index of EGF and EGFR in the granulation tissue of MEBO group was higher than that in the other two groups, and the difference between them was significant in statistics (P < 0. 05). Conclusion MEBO can increase the expression of EGF and EGFR in granulation tissue of chronic skin ulcerative wound and thereby pro-mote the healing of chronic skin ulcer.