CAJ | 학술논문

目的探讨放射线照射早期对Balb/c小鼠畸变产物耳声发射(DPOAE)和耳蜗外毛细胞Prestin蛋白的影响及放射线照射导致感音神经性耳聋的可能机制.方法建立放疗早期感音神经性耳聋(sensorineural hearing loss,SNHL)Balb/c小鼠模型.检测一次性给予单侧耳总放射剂量为16 Gy的放射线之前、照射后第3天、照射后第7天小鼠畸变产物耳声发射幅值,比较放射线照射前后小鼠听觉生理功能的变化情况.提取小鼠耳蜗组织蛋白及mRNA,通过Western blot及逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)方法检测各组小鼠耳蜗Prestin蛋白及其基因Prestin mRNA的表达.结果成功建立了放疗早期SNHL Balb/c小鼠模型.照射后第3天组及照射后第7天组小鼠在3、4、6、8、10和12 kHz时的DPOAE幅值较照射前组小鼠均降低,照射后第7天组小鼠的DPOAE幅值在大部分频率上要比照射后第3天组小鼠的DPOAE幅值要低.照射后第3天组及照射后第7天组小鼠的耳蜗Prestin蛋白及其基因Prestin mRNA的表达较照射前组小鼠出现一致性下降,照射后第7天组小鼠的耳蜗Prestin蛋白及其基因Prestin mRNA的表达较照射后第3天组小鼠下调.结论放射线照射早期即可影响小鼠的听觉生理功能.放射线照射早期可使耳蜗外毛细胞Prestin蛋白及其基因Prestin mRNA的表达下调.本研究表明放射线照射导致SNHL可能与放射线照射引起耳蜗外毛细胞Prestin蛋白功能表达的改变有关.
목적탐토방사선조사조기대Balb/c소서기변산물이성발사(DPOAE)화이와외모세포Prestin단백적영향급방사선조사도치감음신경성이롱적가능궤제.방법건립방료조기감음신경성이롱(sensorineural hearing loss,SNHL)Balb/c소서모형.검측일차성급여단측이총방사제량위16 Gy적방사선지전、조사후제3천、조사후제7천소서기변산물이성발사폭치,비교방사선조사전후소서은각생리공능적변화정황.제취소서이와조직단백급mRNA,통과Western blot급역전록취합매련반응(reverse transcription-polymerase chain reaction,RT-PCR)방법검측각조소서이와Prestin단백급기기인Prestin mRNA적표체.결과성공건립료방료조기SNHL Balb/c소서모형.조사후제3천조급조사후제7천조소서재3、4、6、8、10화12 kHz시적DPOAE폭치교조사전조소서균강저,조사후제7천조소서적DPOAE폭치재대부분빈솔상요비조사후제3천조소서적DPOAE폭치요저.조사후제3천조급조사후제7천조소서적이와Prestin단백급기기인Prestin mRNA적표체교조사전조소서출현일치성하강,조사후제7천조소서적이와Prestin단백급기기인Prestin mRNA적표체교조사후제3천조소서하조.결론방사선조사조기즉가영향소서적은각생리공능.방사선조사조기가사이와외모세포Prestin단백급기기인Prestin mRNA적표체하조.본연구표명방사선조사도치SNHL가능여방사선조사인기이와외모세포Prestin단백공능표체적개변유관.
Objective To study the effect of radiation exposure on the distortion product otoacoustic emission(DPOAE) and Prestin expression in the cochlear outer hair cells of Balb/c mice in the early period after single-dose radiation exposure and to examine the possible mechanism of radiation causing sensorineural hearing loss(SNHL). Methods The Balb/c mouse models with outer hair cells exposed to radiation were established. The DPOAE amplitudes of the mice were detected and compared before, 3 days and 7 days after exposure to a single dose of 16Gy radiation. The Western Blot and reverse transcription-polymerase chain reaction(RT-PCR) assays were used to analyze the changes of expression of Prestin and its mRNA in the cochleae of mice. Results The Balb/c mouse models with SNHL after radiation exposure were successfully established. The DPOAE amplitudes of the mice on the 3rd and 7th day after radiation exposure were lower than those before radiation exposure at 3,4,6,8,10 and 12 kHz and the amplitudes on the 7th day were lower than those on the 3rd day at most frequencies. The expressions of Prestin and its mRNA after radiation exposure were parallel down-regulated as compared with those before exposure and the expressions of Prestin and its mRNA on the 7th day after radiation exposure were lower than those on the 3rd day. Conclusion The auditory function of the mice was affected in the early period after radiation exposure. The mechanism of SNHL after radiation exposure may be correlated with Prestin expression abnormalities in the outer hair cells of cochleae in the early period after radiation exposure.