荧光定量PCR法在一起急性胃肠炎疫情病原检测中的应用
형광정량PCR법재일기급성위장염역정병원검측중적응용
Application of Real-time RT-PCR in the Pathogenic Diagnosis of An Acute Gastroenteritis Outbreak
  • 万方数据
    中国血液流变学杂志 중국혈액류변학잡지
    CHINESE JOURNAL OF HEMORHEOLOGY
    2012年 4期 691-692 ,共2页

    姚萍%李琼*%黎俊宏

    요평%리경*%려준굉

    诺如病毒%荧光定量PCR%急性胃肠炎 諾如病毒%熒光定量PCR%急性胃腸炎
    낙여병독%형광정량PCR%급성위장염
    norovirus%Real-time PCR%acute gastroenteritis
    目的探讨荧光定量PCR法在一起急性胃肠炎疫情病原检测中的应用.方法通过收集常州市某小学暴发急性胃肠炎病例的肛拭子、呕吐物和腹泻物等,运用荧光定量PCR法检测样品中诺如病毒核酸的表达.结果在送检的36例样品中检出诺如病毒GⅡ型15例.结论该次群体性急性胃肠炎暴发疫情病原体为诺如病毒.
    목적탐토형광정량PCR법재일기급성위장염역정병원검측중적응용.방법통과수집상주시모소학폭발급성위장염병례적항식자、구토물화복사물등,운용형광정량PCR법검측양품중낙여병독핵산적표체.결과재송검적36례양품중검출낙여병독GⅡ형15례.결론해차군체성급성위장염폭발역정병원체위낙여병독.
    Objective To determine the pathogen of an unexplained epidemic event of acute gastroenteritis by a method of Real-time RT-PCR.Methods Norovirus was tested by Real-time PCR in the samples from rectal swabs,vomit and diarrhea samples in a primary school of Changzhou city.Results Norovirus GⅡwas detected positively in 15 of 36 samples.Conclusion The epidemic event of acute gastroenteritis was caused by norovirus.
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