CAJ | 학술논문

选用干旱敏感型栽培烟草品种中烟100旺长后期的叶片和感黑胫病栽培烟草品种小黄金1025苗期的叶片作为实验材料,总蛋白质提取分别采用稍加改进的 TCA/丙酮法和由蛋白质分级提取、酚抽提及丙酮洗涤相结合的方法.对中烟100旺长后期烟草叶片采用的总蛋白质 TCA/丙酮提取方法进行了优化、对其使用的 IPG 胶条 pH 范围做了初步筛选,并在此研究基础上,对采用第二种提取方法获得的小黄金1025苗期烟草叶片总蛋白质样品进行了不同上样量对双向电泳结果影响的比较实验.初步建立起适合烟草叶片蛋白质组研究的双向电泳实验体系.
선용간한민감형재배연초품충중연100왕장후기적협편화감흑경병재배연초품충소황금1025묘기적협편작위실험재료,총단백질제취분별채용초가개진적 TCA/병동법화유단백질분급제취、분추제급병동세조상결합적방법.대중연100왕장후기연초협편채용적총단백질 TCA/병동제취방법진행료우화、대기사용적 IPG 효조 pH 범위주료초보사선,병재차연구기출상,대채용제이충제취방법획득적소황금1025묘기연초협편총단백질양품진행료불동상양량대쌍향전영결과영향적비교실험.초보건립기괄합연초협편단백질조연구적쌍향전영실험체계.
The plant materials used in these experiments were the flourishing late tobacco leaves of Zhongyan 100, a drought-sensitive variety, and the seedling leaves of Xiaohuangjin 1025, a variety susceptible for the tobacco black shank pathogen. Total proteins were extracted by trichloroacetic acid / acetone precipitation method, respectively, and protein fractionated extraction combined with phenol extraction and acetone washing method with some minor modifications. A trichloroacetic acid / acetone precipitation method, used for protein extraction from Zhongyan 100 leaves, was optimized. The immobilized pH gradient strips applied to Zhongyan 100 total proteins was selected preliminarily. On the basis of this study, we compared the effects of different protein quantities, from Xiaohuangjin 1025 total proteins extracted by the second protein extraction method, on 2-DE profile. 2-DE experiment protocol suitable for tobacco leaves proteome study was established preliminarily.