CAJ | 학술논문

考察了诃子提取物及其色谱纯化物(a、b、c)清除自由基的活性能力,进行了1,1-二苯基-2-苦肼基(DPPH·)清除试验.结果表明,甲醇提取物、乙酸乙酯提取物及其色谱纯化物均具有清除自由基活性的能力,甲醇粗提物活性略高于等体积1 mg/mL TBHQ,而其色谱纯组分 c 的活性最高,约为1 mg/mL TBHQ 的1.2倍,且略高于茶多酚;乙酸乙酯组分色谱纯组分 a,b 抑制率分别为其粗提取物的1.6和1.1倍.色谱纯成分经显色反应、UV 和 H1NMR 检测后被确认为诃黎勒酸衍生物或诃子单宁水解碎片,此为诃子提取物作为烟用自由基清除剂提供了有效的理论依据.
고찰료가자제취물급기색보순화물(a、b、c)청제자유기적활성능력,진행료1,1-이분기-2-고정기(DPPH·)청제시험.결과표명,갑순제취물、을산을지제취물급기색보순화물균구유청제자유기활성적능력,갑순조제물활성략고우등체적1 mg/mL TBHQ,이기색보순조분 c 적활성최고,약위1 mg/mL TBHQ 적1.2배,차략고우다다분;을산을지조분색보순조분 a,b 억제솔분별위기조제취물적1.6화1.1배.색보순성분경현색반응、UV 화 H1NMR 검측후피학인위가려륵산연생물혹가자단저수해쇄편,차위가자제취물작위연용자유기청제제제공료유효적이론의거.
The free-radical-scavenging activity of different groups from terminalia chebula ritz were studied. The results showed that all extracts of terminalia chebula ritz and three chromatographically purified components had strong DPPH· scavenging effects. The methanol extract in 1 mg/mL showed that it had better free-radical-scavenging activity than that of TBHQ and its chromatographically purified component C showed the strongest activity among all samples. Its activity was slightly stronger than that of tea polyphenol, as 1.2 folds of TBHQ’s. The activities of two chromatographically purified components from ethyl acetate extract were 1.6 and 1.1 folds as the original, respectively. By color test, UV and H1NMR all of the purified components were confirmed to be the derivatives of myrobalani-tannin or their hydrolyzed segments. The extracts of terminalia chebula ritz as free-radical-scavenging of tobacco provided an effective theoretical basis.