中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2013年
1期
72-76
,共5页
烟草%青枯病%室内毒力测定%药剂防治试验%药效评价
煙草%青枯病%室內毒力測定%藥劑防治試驗%藥效評價
연초%청고병%실내독력측정%약제방치시험%약효평개
tobacco%bacterial wilt%allied toxicity measurement%chemical control test%efficiency evaluation
为验证抗生素对烟草青枯病的防治效果,采用纸碟法测定了14种药剂对青枯菌的抑菌活性,水培法测定了5种药剂的内吸防病能力,田间小区试验法测定了9种药剂的田间药效.结果表明,硫酸链霉素,98%金霉素和90%链?土霉素的200单位抑菌活性较强,抑菌圈直径均值16 mm;99%醋酸铜和99%硫酸铜的1000倍液较弱,均值为6.7 mm.98%金霉素和90%链?土霉素的200单位溶液里烟草根吸收预防叶片剪口侵染的青枯病效果分别达100%和97.2%,且有一定的田间防治效果,但9种药剂之间无显著差异.短时间内(10 d)链?铜复配II(硫酸链霉素200单位+53.8%氢氧化铜1000倍液),防效可达100%,随着时间的推移(30 d后),防效消失.但在增施有机质的基础上,配合施用Cu2+或Ca2+,可以提高链霉素的药效.
為驗證抗生素對煙草青枯病的防治效果,採用紙碟法測定瞭14種藥劑對青枯菌的抑菌活性,水培法測定瞭5種藥劑的內吸防病能力,田間小區試驗法測定瞭9種藥劑的田間藥效.結果錶明,硫痠鏈黴素,98%金黴素和90%鏈?土黴素的200單位抑菌活性較彊,抑菌圈直徑均值16 mm;99%醋痠銅和99%硫痠銅的1000倍液較弱,均值為6.7 mm.98%金黴素和90%鏈?土黴素的200單位溶液裏煙草根吸收預防葉片剪口侵染的青枯病效果分彆達100%和97.2%,且有一定的田間防治效果,但9種藥劑之間無顯著差異.短時間內(10 d)鏈?銅複配II(硫痠鏈黴素200單位+53.8%氫氧化銅1000倍液),防效可達100%,隨著時間的推移(30 d後),防效消失.但在增施有機質的基礎上,配閤施用Cu2+或Ca2+,可以提高鏈黴素的藥效.
위험증항생소대연초청고병적방치효과,채용지설법측정료14충약제대청고균적억균활성,수배법측정료5충약제적내흡방병능력,전간소구시험법측정료9충약제적전간약효.결과표명,류산련매소,98%금매소화90%련?토매소적200단위억균활성교강,억균권직경균치16 mm;99%작산동화99%류산동적1000배액교약,균치위6.7 mm.98%금매소화90%련?토매소적200단위용액리연초근흡수예방협편전구침염적청고병효과분별체100%화97.2%,차유일정적전간방치효과,단9충약제지간무현저차이.단시간내(10 d)련?동복배II(류산련매소200단위+53.8%경양화동1000배액),방효가체100%,수착시간적추이(30 d후),방효소실.단재증시유궤질적기출상,배합시용Cu2+혹Ca2+,가이제고련매소적약효.
In order to validate the control effect of antibiotics to Tobacco Bacterial Wilt, fourteen bactericides were employed to evaluate the antibacterial activity to the Ralstonia solanacearum with a paper disk test, and five chemicals were applied to survey the preventing ability of disease through systemic infection by the method of water culture and leaf-cutting, the nine chemicals were utilized to study their control effects to bacterial wilt in field. The results indicated that the 200 unit streptomycin sulfate, chlortetracycline and streptomycin+oxytetracycline showed the stronger antibacterial activity with the average inhibition zone 16 mm in diameter, which was much better than the 1000 times liquid of copper acetate and copper sulfate whose average inhibition zone were 6.7 mm in diameter. The control effects of 200 unit chlortetracycline and chain oxytetracycline reached to 100% and 97.2% through root absorption to prevent the bacterial wilt caused by leaf-cutting of tobacco leaves. There was no significant difference among 9 kinds of bactericides. In a short time (10 d), the field control effects of 200 unit streptomycin sulfate+1000 times liquid of 53.8%copper sulfate reached to 100%, but this effects disappeared after 30 d. Application of organic fertilizers combined with Cu2+or Ca2+application can improve the efficacy of streptomycin.