CAJ | 학술논문

采用以烟碱作为唯一碳氮源的选择性培养基,对烟草表面的细菌进行选择性分离,建立降低烟碱含量菌库,通过富集初筛复筛等试验后,从中筛选出一株具有高效降解烟碱能力的细菌MK21.根据形态学特征和生理生化反应及16SrDNA的鉴定,鉴定其为短小芽孢杆菌.将该菌制成菌剂喷施于50 g烟丝样品上,发酵处理5 d.通过测定烟丝的总糖、还原糖、烟碱、氯、钾含量.结果表明:处理后的烟丝中烟碱含量均有下降,其中最高的达到了26.3%.通过短小芽孢杆菌MK21处理后的烟叶样品,达到刺激性降低、掩盖杂气和改善卷烟吸味的效果.
채용이연감작위유일탄담원적선택성배양기,대연초표면적세균진행선택성분리,건립강저연감함량균고,통과부집초사복사등시험후,종중사선출일주구유고효강해연감능력적세균MK21.근거형태학특정화생리생화반응급16SrDNA적감정,감정기위단소아포간균.장해균제성균제분시우50 g연사양품상,발효처리5 d.통과측정연사적총당、환원당、연감、록、갑함량.결과표명:처리후적연사중연감함량균유하강,기중최고적체도료26.3%.통과단소아포간균MK21처리후적연협양품,체도자격성강저、엄개잡기화개선권연흡미적효과.
Study was carried out in order to provide a theoretical basis for research and application of reducing nicotine by microbial. Nicotine was chosen as the only selective medium of nitrogen source. A series of bacteria which can decompose nicotine were found, and a strain coded MK21 was identified. According to its morphology feature and physiological and biochemical characteristics and test on 16SrDNA, Breibacillus breis was identified Applying this bacteria on 50g samples of cut tobacco and then leave for fermentation for 5 days, Content of total sugar, reducing sugar, nicotine, chlorine and potassium were measured. Results showed that nicotine content in all samples declined as far as to 26.3%. Samples treated by MK21 with lower irritating chemicals can cover unpleasantness and improve taste.