CAJ | 학술논문

从广东凡口铅锌矿尾矿库周边土壤中分离出一株能高效降解松醇油的细菌 KS-1,并进行人工配制含松醇油的模拟废水的化学需氧量(COD)降低条件优化试验研究.形态学和生理学分析表明:该 KS-1菌株为革兰氏阳性好氧菌,呈细胞杆状、芽孢椭圆或柱状;接触酶、硝酸盐还原和 V-P 测定呈阳性反应;能利用葡萄糖、木糖、甘露醇、柠檬酸盐、淀粉和明胶生长,不能利用吲哚、卵黄卵磷脂酶、苯丙氨酸脱氨酶和丙酸盐生长.16SrDNA序列分析表明:该菌株与 Bacillus subtilis AF0907的相似性达到100%,最终鉴定菌株 KS-1为枯草芽孢杆菌(Bacillus subtilis AF0907),登录号为 GU272021.正交试验优化结果表明:菌株 KS-1降低松醇油废水 COD 工艺最佳条件为 pH4,接种量15%,摇床转速200 r/min,松醇油浓度300 mg/L,在此条件下反应4 d,COD 去除率达到65.91%.
종엄동범구연자광미광고주변토양중분리출일주능고효강해송순유적세균 KS-1,병진행인공배제함송순유적모의폐수적화학수양량(COD)강저조건우화시험연구.형태학화생이학분석표명:해 KS-1균주위혁란씨양성호양균,정세포간상、아포타원혹주상;접촉매、초산염환원화 V-P 측정정양성반응;능이용포도당、목당、감로순、저몽산염、정분화명효생장,불능이용신타、란황란린지매、분병안산탈안매화병산염생장.16SrDNA서렬분석표명:해균주여 Bacillus subtilis AF0907적상사성체도100%,최종감정균주 KS-1위고초아포간균(Bacillus subtilis AF0907),등록호위 GU272021.정교시험우화결과표명:균주 KS-1강저송순유폐수 COD 공예최가조건위 pH4,접충량15%,요상전속200 r/min,송순유농도300 mg/L,재차조건하반응4 d,COD 거제솔체도65.91%.
A strain KS-1 capable of highly degrading pine oil was isolated from the soil collected from tailings reservoir in Fankou Lead-zinc Mine of Guangdong province, and the best degradation conditions of pine oil wastewater (chemical oxygen demand, COD) were investigated. The analysis of morphology and physiology shows that the strain KS-1 is gram-positive and aerobic bacterium and rhabditiform, which can grow by using glucose, xylose, mannitol, citrate, starch and gelatin and cannot grow with indole, yolk lecithinase, phenylalanine deaminase and propionate. Based on analysis of phenotype, physiological and biochemical characters and 16SrDNA, KS-1 is identified as Bacillus subtilis AF0907 due to its 16SrDNA sequence, GU272021, which has the similarity of 100% to Bacillus subtilis AF0907. According to the orthogonal optimization test, the degradation conditions are as follows: pH 4, inoculating ratio 15%, rotation speed 200 r/min, pine oil concentration 300 mg/L, with COD removal rate up to 65.91% in 4 d.