中国药物应用与监测
中國藥物應用與鑑測
중국약물응용여감측
CHINESE JOURNAL OF DRUG APPLICATION AND MONITORING
2013年
1期
17-20
,共4页
高效液相色谱法%感冒清热颗粒%葛根素%胡薄荷酮%含量测定
高效液相色譜法%感冒清熱顆粒%葛根素%鬍薄荷酮%含量測定
고효액상색보법%감모청열과립%갈근소%호박하동%함량측정
HPLC%Ganmao qingre granules%Puerarin%Pulegone%Content determination
目的:建立HPLC法同时测定感冒清热颗粒中葛根素与胡薄荷酮含量的方法.方法:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),用甲醇-水梯度洗脱,检测波长252 nm,柱温40℃,流速1.0 mL·min-1,进样量10μL.结果:葛根素、胡薄荷酮与其他杂质峰分离良好.葛根素在5.532~88.512μg·mL-1与峰面积呈良好的线性关系(r =1.0000);胡薄荷酮在0.786~12.573μg·mL-1与峰面积呈良好的线性关系(r=0.9999),葛根素、胡薄荷酮平均加样回收率分别为100.73%和99.78%,RSD分别为0.62%(n=6)和0.75%(n=6);不同厂家生产的感冒清热颗粒中葛根素和胡薄荷酮的含量差异较大.结论:该方法操作简便,结果准确,可作为感冒清热颗粒的质量控制方法.
目的:建立HPLC法同時測定感冒清熱顆粒中葛根素與鬍薄荷酮含量的方法.方法:採用Diamonsil C18色譜柱(250 mm×4.6 mm,5μm),用甲醇-水梯度洗脫,檢測波長252 nm,柱溫40℃,流速1.0 mL·min-1,進樣量10μL.結果:葛根素、鬍薄荷酮與其他雜質峰分離良好.葛根素在5.532~88.512μg·mL-1與峰麵積呈良好的線性關繫(r =1.0000);鬍薄荷酮在0.786~12.573μg·mL-1與峰麵積呈良好的線性關繫(r=0.9999),葛根素、鬍薄荷酮平均加樣迴收率分彆為100.73%和99.78%,RSD分彆為0.62%(n=6)和0.75%(n=6);不同廠傢生產的感冒清熱顆粒中葛根素和鬍薄荷酮的含量差異較大.結論:該方法操作簡便,結果準確,可作為感冒清熱顆粒的質量控製方法.
목적:건립HPLC법동시측정감모청열과립중갈근소여호박하동함량적방법.방법:채용Diamonsil C18색보주(250 mm×4.6 mm,5μm),용갑순-수제도세탈,검측파장252 nm,주온40℃,류속1.0 mL·min-1,진양량10μL.결과:갈근소、호박하동여기타잡질봉분리량호.갈근소재5.532~88.512μg·mL-1여봉면적정량호적선성관계(r =1.0000);호박하동재0.786~12.573μg·mL-1여봉면적정량호적선성관계(r=0.9999),갈근소、호박하동평균가양회수솔분별위100.73%화99.78%,RSD분별위0.62%(n=6)화0.75%(n=6);불동엄가생산적감모청열과립중갈근소화호박하동적함량차이교대.결론:해방법조작간편,결과준학,가작위감모청열과립적질량공제방법.
Objective:To establish a method for the simultaneous quantification of puerarin and pulegone in ganmao qingre granules. Methods:Chromatography was performed using a Diamonsil C18 column (250 mm×4.6 mm, 5μm). The mobile phase consisted of methanol and water. The detection wave lengths was 252 nm and column temperature was 40℃. Results:The calibration curves of puerarin and pulegone were linear in the range of 5.532–88.512μg·mL-1 (r=1.000 0) and 0.786–12.573μg·mL-1(r=0.9999) respectively. The average recoveries were 100.73%and 99.78%;the values of RSD were 0.62%(n=6)and 0.75%(n=6). Contents of puerarian in ganmao qingre granules from different manufacturers were different. Conclusion:The method is sensitive, accurate and can be used for the quality control of ganmao qingre granules.
