CAJ | 학술논문

目的:研究广西地区乙肝病毒基因型分布及与临床表现及机体免疫功能的关系.方法:收集广西地区原籍人口不同病情的慢性乙肝病毒相关性疾病包括无症状携带者(ASC)、慢性乙型病毒性肝炎(CHB)、乙肝后肝硬化(LC)、原发性肝癌(HCC)血液标本,检测乙肝病毒血清学标志物(HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc 和抗-HBcIgM),HBVDNA 拷贝数,肝功能,T 淋巴细胞亚群,型特异性引物巢式 PCR 及测序鉴定病毒基因型,利用统计软件 SPSS 17.0进行资料分析.结果:本研究检测纳入的研究对象感染乙肝病毒为B、C 两种基因型,未检测到其他基因型,以 C 型(66.3%)为主,B 型(33.7%)次之.C 基因型的 HBeAg 血清转换率低于 B 型(P<0.05).C 型在肝癌组的比例显著高于其他疾病组(P<0.05),不同基因型间年龄、HBVDNA 拷贝数、AST、ALT、T 细胞亚群 CD3(+)、CD4(+)、CD8(+)的差异无统计学意义.结论:广西地区乙肝病毒感染相关性肝病患者 HBV 基因型为 B 型、C 型,以 C 型为主,C 型与较严重病情有关,感染不同 HBV 基因型与机体的免疫状况无明显相关.
목적:연구엄서지구을간병독기인형분포급여림상표현급궤체면역공능적관계.방법:수집엄서지구원적인구불동병정적만성을간병독상관성질병포괄무증상휴대자(ASC)、만성을형병독성간염(CHB)、을간후간경화(LC)、원발성간암(HCC)혈액표본,검측을간병독혈청학표지물(HBsAg、항-HBs、HBeAg、항-HBe、항-HBc 화항-HBcIgM),HBVDNA 고패수,간공능,T 림파세포아군,형특이성인물소식 PCR 급측서감정병독기인형,이용통계연건 SPSS 17.0진행자료분석.결과:본연구검측납입적연구대상감염을간병독위B、C 량충기인형,미검측도기타기인형,이 C 형(66.3%)위주,B 형(33.7%)차지.C 기인형적 HBeAg 혈청전환솔저우 B 형(P<0.05).C 형재간암조적비례현저고우기타질병조(P<0.05),불동기인형간년령、HBVDNA 고패수、AST、ALT、T 세포아군 CD3(+)、CD4(+)、CD8(+)적차이무통계학의의.결론:엄서지구을간병독감염상관성간병환자 HBV 기인형위 B 형、C 형,이 C 형위주,C 형여교엄중병정유관,감염불동 HBV 기인형여궤체적면역상황무명현상관.
Objective:To investigate the distribution and virologic features of HBV genotypes in GuangXi province and the association of the clinical manifestation.Method:Serum samples were collected from asymptomatic carrier(ASC),liver cirrhosis(LC),chronic hepatitis B(CHB), primary hepatic cell carcinoma(HCC)individuals. Liver function,biochemical markers and T-lymphcyte subpopulations were detected.Type-specific primers nested polymerase chain reaction was applied for HBV genotyping. The data was analyzed with SPSS 17.0.Result:Genotype C was found to be predominant(66.3%)among the studied samples,followed by genotype B(33.7%).HBeAg serum conversion rate of C genotype was significantly lower than that of B genotype,while the proportion of C genotype in HCC group exhibited significantly higher than that in ASC,CHB and LC groups.No significant difference was found in age,viral loads,ALT,AST and T cell subsets between B,C genotypes.Conclusion:There are B and C genotypes prevalent in GuangXi,with C being significantly dominant. Genotype C is associated with more severe liver diseases. The association between the virus genotype and the cellular immunity remain a field to be explored.