中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
2期
7-9
,共3页
洪妙玲%黄伟青%刘佩仪%武钊%文锐玲%郭兰英%黄瑞娇%欧阳斌%郭伟%梁庆
洪妙玲%黃偉青%劉珮儀%武釗%文銳玲%郭蘭英%黃瑞嬌%歐暘斌%郭偉%樑慶
홍묘령%황위청%류패의%무쇠%문예령%곽란영%황서교%구양빈%곽위%량경
DADLE%全脑缺血再灌注%ERK%Caspase-3
DADLE%全腦缺血再灌註%ERK%Caspase-3
DADLE%전뇌결혈재관주%ERK%Caspase-3
DADLE%Global cerebral ischemia-reperfusion%ERK%Caspase-3
目的:观察δ-阿片受体激动剂 DADLE([D-Ala2,D-Leu5]enkephali)对大鼠急性全脑缺血再灌注后海马区神经元 ERK 信号通路和 Caspase-3表达的影响.方法:将50只 SD 大鼠随机分为5组:假手术组(Sham)、模型组(I/R)、DADLE 处理组(根据不同剂量可分为2 mg/kg[A]、3 mg/kg[B]、5 mg/kg[C]).采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型.于缺血15 min 后经颈外静脉注射 DADLE 并于再灌注120 min 后处死.开颅取其新鲜海马组织,采用 western blot 检测海马组织 Caspase-3的表达,以及采用免疫组织化学法检测非磷酸化 ERK 与磷酸化 ERK(P-ERK)的表达状况.结果:Sham 组 ERK 和 P-ERK 蛋白表达水平显著低于其他各组(P<0.05), DADLE 处理组与 I/R 组相比,其 ERK 和 P-ERK 蛋白的表达量明显上调(P<0.05).海马组织 Caspase-3蛋白表达 I/R 组较 Sham 组表达明显上调(P<0.05),而 DADLE 处理组与 IR 组比较,海马组织 Caspase-3蛋白表达明显下降(P<0.05).结论:DADLE 对大鼠急性全脑缺血再灌注后海马区神经元有保护作用,说明 DADLE 可通过上调 ERK 信号通路以及抑制 Caspase-3的表达而起到保护脑组织的作用.
目的:觀察δ-阿片受體激動劑 DADLE([D-Ala2,D-Leu5]enkephali)對大鼠急性全腦缺血再灌註後海馬區神經元 ERK 信號通路和 Caspase-3錶達的影響.方法:將50隻 SD 大鼠隨機分為5組:假手術組(Sham)、模型組(I/R)、DADLE 處理組(根據不同劑量可分為2 mg/kg[A]、3 mg/kg[B]、5 mg/kg[C]).採用改良的二血管阻斷加低血壓法建立全腦缺血再灌註模型.于缺血15 min 後經頸外靜脈註射 DADLE 併于再灌註120 min 後處死.開顱取其新鮮海馬組織,採用 western blot 檢測海馬組織 Caspase-3的錶達,以及採用免疫組織化學法檢測非燐痠化 ERK 與燐痠化 ERK(P-ERK)的錶達狀況.結果:Sham 組 ERK 和 P-ERK 蛋白錶達水平顯著低于其他各組(P<0.05), DADLE 處理組與 I/R 組相比,其 ERK 和 P-ERK 蛋白的錶達量明顯上調(P<0.05).海馬組織 Caspase-3蛋白錶達 I/R 組較 Sham 組錶達明顯上調(P<0.05),而 DADLE 處理組與 IR 組比較,海馬組織 Caspase-3蛋白錶達明顯下降(P<0.05).結論:DADLE 對大鼠急性全腦缺血再灌註後海馬區神經元有保護作用,說明 DADLE 可通過上調 ERK 信號通路以及抑製 Caspase-3的錶達而起到保護腦組織的作用.
목적:관찰δ-아편수체격동제 DADLE([D-Ala2,D-Leu5]enkephali)대대서급성전뇌결혈재관주후해마구신경원 ERK 신호통로화 Caspase-3표체적영향.방법:장50지 SD 대서수궤분위5조:가수술조(Sham)、모형조(I/R)、DADLE 처리조(근거불동제량가분위2 mg/kg[A]、3 mg/kg[B]、5 mg/kg[C]).채용개량적이혈관조단가저혈압법건립전뇌결혈재관주모형.우결혈15 min 후경경외정맥주사 DADLE 병우재관주120 min 후처사.개로취기신선해마조직,채용 western blot 검측해마조직 Caspase-3적표체,이급채용면역조직화학법검측비린산화 ERK 여린산화 ERK(P-ERK)적표체상황.결과:Sham 조 ERK 화 P-ERK 단백표체수평현저저우기타각조(P<0.05), DADLE 처리조여 I/R 조상비,기 ERK 화 P-ERK 단백적표체량명현상조(P<0.05).해마조직 Caspase-3단백표체 I/R 조교 Sham 조표체명현상조(P<0.05),이 DADLE 처리조여 IR 조비교,해마조직 Caspase-3단백표체명현하강(P<0.05).결론:DADLE 대대서급성전뇌결혈재관주후해마구신경원유보호작용,설명 DADLE 가통과상조 ERK 신호통로이급억제 Caspase-3적표체이기도보호뇌조직적작용.
Objective:To observe the effects of δ-opioid receptor agonist DADLE([D-Ala2,D-Leu5]enkephali)on expression of ERK signaling pathway and Caspase-3 following cerebral ischemia-reperfusion in hippocampus of rat. Method:In total,50 SD rats were randomly divided into 5 groups:a sham-operated,a I/R and three DADLE-treated(different drug doses:2 mg/kg[A],3 mg/kg[B] and 5 mg/kg[C]).Use improvement of two vessels occlusion add hypotension to establish the global cerebral ischemia/reperfusion model in rats.Injecting DADLE through external jugular vein after ischemic 15 min,and the rats were respectively killed after reperfusion 120min.The expression of Caspase-3 were detected by Western blot,and the expression of non-phosphorylated ERK and phosphorylation of ERK(P-ERK)were detected by Immunohistochemistry assay. Result:The expression of ERK and P-ERK was lower in the Sham group than in the others(P<0.05),and compared with I/R group,the expression of ERK and P-ERK of each DADLE-treated group was higher obviously(P<0.05). The expression of Caspase-3 in hippocampus was higher in the I/R group than in the Sham group, and compared with I/R group,the expression of Caspase-3 of each DADLE-treated group was lower obviously(P<0.05). Conclusion:DADLE may be have some protective effects on neurons in hippocampus after the global cerebral ischemia/reperfusion in rats,and it related. Shows that DADLE can play a role in protecting brain tissue by increasing the expression of ERK signaling pathway and inhibiting the expression of Caspase-3.
