CAJ | 학술논문

目的:研究 syndecan-1在结肠腺瘤及腺癌中的表达,探讨其与结肠腺瘤及结肠癌的关系.方法:分别采用免疫组织化学染色技术(immunohistochemistry,IHC)以及逆转录-聚合酶链反应技术(reverse transcription polymerase chain reaction,RT-PCR),检测结肠腺瘤、腺癌及正常肠道组织中 syndecan-1 mRNA 和蛋白的表达,初步分析其与腺癌的临床病理特征的关系.结果:免疫组化及 RT-PCR 均显示,syndecan-1在正常组织中明显高表达,而腺癌呈明显低表达,腺瘤则介于两者之间,三者之间差异有统计学意义(P<0.05).结论:syndecan-1主要表达于正常肠黏膜组织,在腺瘤中表达有所减弱,在腺癌中的表达则明显减弱.这提示细胞间粘附的破坏在结直肠的致癌机制中可能起关键作用.
목적:연구 syndecan-1재결장선류급선암중적표체,탐토기여결장선류급결장암적관계.방법:분별채용면역조직화학염색기술(immunohistochemistry,IHC)이급역전록-취합매련반응기술(reverse transcription polymerase chain reaction,RT-PCR),검측결장선류、선암급정상장도조직중 syndecan-1 mRNA 화단백적표체,초보분석기여선암적림상병리특정적관계.결과:면역조화급 RT-PCR 균현시,syndecan-1재정상조직중명현고표체,이선암정명현저표체,선류칙개우량자지간,삼자지간차이유통계학의의(P<0.05).결론:syndecan-1주요표체우정상장점막조직,재선류중표체유소감약,재선암중적표체칙명현감약.저제시세포간점부적파배재결직장적치암궤제중가능기관건작용.
Objective:To investigate the expression of syndecan-1 on colorectal adenoma and adencarcinoma,and to explore its relationship to the progression of adenoma and cancer.Method:Immunohistochemistry and reverse transcription-PCR were used to detect the expression of syndecan-1 mRNA and protein in adenomas,adencarcinomasand normal intestinal tissues. Its expression was evaluated with the relationship to clinicopathological factors.Result:Both immunohistochemistry and RT-PCR showed that syndecan-1 was highly expressed in normal tissues and was lowly expressed in adencarcinomas,and was middly expressed in adenoma.All of these had significant differences(P<0.05).Conclusion:Syndecan-1 is expressed in normal tissue,whilethe expression was lower in adenomas and much lower in adencarcinomas.It is indicated that the destroy of adherence may play a key role in carcinogenesis.