中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
7期
91-92
,共2页
尿铅测定%石墨炉原子吸收光谱法%基体改进剂%氯化钯
尿鉛測定%石墨爐原子吸收光譜法%基體改進劑%氯化鈀
뇨연측정%석묵로원자흡수광보법%기체개진제%록화파
Urinary phpalbum determination%GFAAS%Matrix modifier%Palladium chloride
目的:探讨钯作为基体改进剂在石墨炉原子吸收光谱法测定尿铅中的应用价值.方法:用氯化钯作为基体改进剂,配制标准曲线,然后对非铅接触组和铅接触组尿液测定,用同法对尿铅标样进行测定,观察其相对误差.结果:标准1(尿比重≤1.016)的标准曲线为 Y=0.746X+0.04816(r=0.9999);标准2(尿比重1.016~1.028)为 Y=0.988X-0.03418(r=0.9999);标准3(尿比重>1.028)为Y=O.948X+0.05346(r=0.9999);三个标准曲线铅的最低检出浓度均为2.0μg/L.精密度实验中,平均浓度为(26.75±1.19)μg/L、(73.24±1.33)μg/L 的两尿样,RSD 分别为5.27%、3.47%.准确度试验中,浓度为30.26、50.26、70.26μg/L 的加标尿样测得的回收率分别为102.28%、98.55%、97.62%.用该法测定尿铅标样 ZK103-1(82.00μg/L)、ZK103-2(221.00μg/L)相对误差分别为1.70%、1.41%.用该法均可测定正常人及铅接触人群的尿铅浓度.结论:石墨炉原子吸收光谱法应用基体改进剂测定尿铅速度快,准确度、灵敏度高,结果可靠.
目的:探討鈀作為基體改進劑在石墨爐原子吸收光譜法測定尿鉛中的應用價值.方法:用氯化鈀作為基體改進劑,配製標準麯線,然後對非鉛接觸組和鉛接觸組尿液測定,用同法對尿鉛標樣進行測定,觀察其相對誤差.結果:標準1(尿比重≤1.016)的標準麯線為 Y=0.746X+0.04816(r=0.9999);標準2(尿比重1.016~1.028)為 Y=0.988X-0.03418(r=0.9999);標準3(尿比重>1.028)為Y=O.948X+0.05346(r=0.9999);三箇標準麯線鉛的最低檢齣濃度均為2.0μg/L.精密度實驗中,平均濃度為(26.75±1.19)μg/L、(73.24±1.33)μg/L 的兩尿樣,RSD 分彆為5.27%、3.47%.準確度試驗中,濃度為30.26、50.26、70.26μg/L 的加標尿樣測得的迴收率分彆為102.28%、98.55%、97.62%.用該法測定尿鉛標樣 ZK103-1(82.00μg/L)、ZK103-2(221.00μg/L)相對誤差分彆為1.70%、1.41%.用該法均可測定正常人及鉛接觸人群的尿鉛濃度.結論:石墨爐原子吸收光譜法應用基體改進劑測定尿鉛速度快,準確度、靈敏度高,結果可靠.
목적:탐토파작위기체개진제재석묵로원자흡수광보법측정뇨연중적응용개치.방법:용록화파작위기체개진제,배제표준곡선,연후대비연접촉조화연접촉조뇨액측정,용동법대뇨연표양진행측정,관찰기상대오차.결과:표준1(뇨비중≤1.016)적표준곡선위 Y=0.746X+0.04816(r=0.9999);표준2(뇨비중1.016~1.028)위 Y=0.988X-0.03418(r=0.9999);표준3(뇨비중>1.028)위Y=O.948X+0.05346(r=0.9999);삼개표준곡선연적최저검출농도균위2.0μg/L.정밀도실험중,평균농도위(26.75±1.19)μg/L、(73.24±1.33)μg/L 적량뇨양,RSD 분별위5.27%、3.47%.준학도시험중,농도위30.26、50.26、70.26μg/L 적가표뇨양측득적회수솔분별위102.28%、98.55%、97.62%.용해법측정뇨연표양 ZK103-1(82.00μg/L)、ZK103-2(221.00μg/L)상대오차분별위1.70%、1.41%.용해법균가측정정상인급연접촉인군적뇨연농도.결론:석묵로원자흡수광보법응용기체개진제측정뇨연속도쾌,준학도、령민도고,결과가고.
Objective:To investigate the application value about palladium as matrix modifier on determination of phpalbum in urine by GFAAS. Method:Palladium chloride as a matrix modifier,standard preparation was curved,and then the non phpalbum exposure group and phpalbum exposure group urine were determinated,and using the same method,urine phpalbum standard samples were determined,observed the relative error. Result:The standard one’s( urine specific gravity was less than or equal to 1.016)standard curve was Y=0.746X+0.04816(r=0.9999);the standard 2’s(urine specific gravity was between 1.016 and 1.028)was Y=0.988X-0.03418(r=0.9999);the standard 3’s(urine specific gravity>1.028)was Y=0.948X+0.05346(r=0.9999);the minimum detectable concentrations of three standard curve were 2 μg/L.In precision experiment,the RSD respectively were 5.27%,3.47% in two urine samples whose average concentration respectively were(26.75 +1.19)μg/L,(73.24 +1.33)μg/L. In accuracy experiment,the recovered rate respectively were 102.28%,98.55%,97.62% in three standard urine samples increased amount whose concentrations respectively were 30.26,50.26,70.26 μg/L.Using the same method,urinary phpalbum standard samples that were ZK103-1(82.00 μg/L), ZK103-2(221.00 μg/L)were determinated,and those relative errors were 1.70%,1.41%.Using the same method,the urine lead concentration of normal persons and lead exposure persons could be determinated.Conclusion:Palladium as matrix modifier,phpalbum in urine is determinated by GFAAS,the method is speedly,its accuracy and sensitivity are high,its result is reliable.
