中国医学影像学杂志
中國醫學影像學雜誌
중국의학영상학잡지
CHINESE JOURNAL OF MEDICAL IMAGING
2012年
12期
924-927
,共4页
神经胶质瘤%超声疗法%声动力疗法%细胞凋亡%大鼠
神經膠質瘤%超聲療法%聲動力療法%細胞凋亡%大鼠
신경효질류%초성요법%성동력요법%세포조망%대서
Glioma%Ultrasonic therapy%Sonodynamic therapy%Apoptosis%Rats
目的探讨声动力疗法对荷瘤鼠脑胶质瘤组织的促凋亡作用及其治疗机制.材料与方法74只 Wistar 大鼠于右侧尾状核处接种 C6胶质瘤细胞,制作颅内胶质瘤模型,行增强 MRI 筛选荷瘤鼠.依 MRI 结果分为对照组、血卟啉甲醚组(HMME 组)、超声组和声动力组(SDT 组),每组16只.声动力参数为1.0MHz、1.0W/cm2、60s 超声辐照和5mg/kg 血卟啉甲醚.治疗后24h 行TUNEL 染色检测胶质瘤细胞凋亡率,电镜检测凋亡形态学改变,免疫组化检测蛋白 GFAP、S-100、Bcl-2/Bax、Caspase-3、Caspase-9、Fas/Fas-L 表达和 Cyt c释放,并记录4组大鼠生存期.结果74只 Wistar 大鼠共68只成瘤,成瘤率为91.89%.与对照组[(3.20±0.72)%]比较,SDT 组[(30.60±1.60)%]及超声组[(14.50±0.80)%]凋亡率显著提高(P<0.05),HMME 组[(3.60±0.51)%]无明显凋亡发生(P>0.05);SDT 组荷瘤鼠生存期长达(46.3±3.1)d,较对照组[(31.1±2.1)d]显著延长(P<0.05),超声组[(32.4±2.3)d]、HMME 组[(30.1±3.0)d]生存期较对照组无明显变化(P>0.05).电镜检测到 SDT 组、超声组胶质瘤细胞核固缩,染色质边集,线粒体肿胀;免疫组化显示 GFAP、S-100、Bax、Caspase-3、Caspase-9呈高表达,Bcl-2、Fas-L 呈低表达和 Cyt c释放,Fas 表达无明显变化.结论声动力疗法对荷瘤鼠胶质瘤有促凋亡治疗作用,并与线粒体内源性凋亡途径密切相关.
目的探討聲動力療法對荷瘤鼠腦膠質瘤組織的促凋亡作用及其治療機製.材料與方法74隻 Wistar 大鼠于右側尾狀覈處接種 C6膠質瘤細胞,製作顱內膠質瘤模型,行增彊 MRI 篩選荷瘤鼠.依 MRI 結果分為對照組、血卟啉甲醚組(HMME 組)、超聲組和聲動力組(SDT 組),每組16隻.聲動力參數為1.0MHz、1.0W/cm2、60s 超聲輻照和5mg/kg 血卟啉甲醚.治療後24h 行TUNEL 染色檢測膠質瘤細胞凋亡率,電鏡檢測凋亡形態學改變,免疫組化檢測蛋白 GFAP、S-100、Bcl-2/Bax、Caspase-3、Caspase-9、Fas/Fas-L 錶達和 Cyt c釋放,併記錄4組大鼠生存期.結果74隻 Wistar 大鼠共68隻成瘤,成瘤率為91.89%.與對照組[(3.20±0.72)%]比較,SDT 組[(30.60±1.60)%]及超聲組[(14.50±0.80)%]凋亡率顯著提高(P<0.05),HMME 組[(3.60±0.51)%]無明顯凋亡髮生(P>0.05);SDT 組荷瘤鼠生存期長達(46.3±3.1)d,較對照組[(31.1±2.1)d]顯著延長(P<0.05),超聲組[(32.4±2.3)d]、HMME 組[(30.1±3.0)d]生存期較對照組無明顯變化(P>0.05).電鏡檢測到 SDT 組、超聲組膠質瘤細胞覈固縮,染色質邊集,線粒體腫脹;免疫組化顯示 GFAP、S-100、Bax、Caspase-3、Caspase-9呈高錶達,Bcl-2、Fas-L 呈低錶達和 Cyt c釋放,Fas 錶達無明顯變化.結論聲動力療法對荷瘤鼠膠質瘤有促凋亡治療作用,併與線粒體內源性凋亡途徑密切相關.
목적탐토성동력요법대하류서뇌효질류조직적촉조망작용급기치료궤제.재료여방법74지 Wistar 대서우우측미상핵처접충 C6효질류세포,제작로내효질류모형,행증강 MRI 사선하류서.의 MRI 결과분위대조조、혈계람갑미조(HMME 조)、초성조화성동력조(SDT 조),매조16지.성동력삼수위1.0MHz、1.0W/cm2、60s 초성복조화5mg/kg 혈계람갑미.치료후24h 행TUNEL 염색검측효질류세포조망솔,전경검측조망형태학개변,면역조화검측단백 GFAP、S-100、Bcl-2/Bax、Caspase-3、Caspase-9、Fas/Fas-L 표체화 Cyt c석방,병기록4조대서생존기.결과74지 Wistar 대서공68지성류,성류솔위91.89%.여대조조[(3.20±0.72)%]비교,SDT 조[(30.60±1.60)%]급초성조[(14.50±0.80)%]조망솔현저제고(P<0.05),HMME 조[(3.60±0.51)%]무명현조망발생(P>0.05);SDT 조하류서생존기장체(46.3±3.1)d,교대조조[(31.1±2.1)d]현저연장(P<0.05),초성조[(32.4±2.3)d]、HMME 조[(30.1±3.0)d]생존기교대조조무명현변화(P>0.05).전경검측도 SDT 조、초성조효질류세포핵고축,염색질변집,선립체종창;면역조화현시 GFAP、S-100、Bax、Caspase-3、Caspase-9정고표체,Bcl-2、Fas-L 정저표체화 Cyt c석방,Fas 표체무명현변화.결론성동력요법대하류서효질류유촉조망치료작용,병여선립체내원성조망도경밀절상관.
Purpose To investigate the apoptotic effect and mechanisms to C6 glioma cells by sonodynamic therapy (SDT) in vivo. Materials and Methods Seventy-four tumor-bearing rats were established by inoculating C6 glioma cells into right caudate nucleus of Wistar rats with stereotactic technique and selected by enhanced MRI. The rats were completely randomized and divided into control group, hematoporphyrin monomethyl ether (HMME) group, ultrasound group and SDT group, 16 rats in each group. The parameters of SDT was ultrasound under 1.0 MHz, 1.0 W/cm2, 60s and HMME of 5mg/kg. After 24 hours by SDT treatment or not, apoptotic rate was determined by TUNEL staining, morphological change was observed by the transmission electron microscope (TEM), protein expression of GFAP, S-100, Bcl-2/Bax, Caspase-3, Caspase-9, Fas/Fas-L and cytochrome (Cyt c) were detected by immunohistochemistry. The rats survival time in four groups was also recorded. Results The SDT group showed the highest apoptotic rate of (30.60±1.60)% (P<0.05), then the ultrasound group of (14.50±0.80)% (P<0.05), and no significant apoptosis was observed in the HMME group when compared to the control group. The long survival time of (46.3±3.1) days was presented in the SDT group (P<0.05), and no obvious change was displayed in survival time in the other three groups. Morphological characteristic changes of apoptosis including nuclear chromatin margination, aggregation and condensation were observed by TEM in SDT group. The expressions of the GFAP, S-100, Caspase-9, Caspase-3, Bax were up-regulated and release of Cyt c, Bcl-2 and Fas-L were down-regulated, and Fas was constant in tumor-bearing rats in the SDT group compared to the other three groups. Conclusion SDT has an obviously therapeutic effect to C6 glima cells in rats in vivo, and is closely associated with the mitochondrial signal apoptosis pathway..
