CAJ | 학술논문

目的:研究口腔扁平苔藓中不同类型角蛋白质的表达及其临床意义.方法:选择口腔扁平苔藓患者,选择扁平苔藓标本作为观察组、正常颊粘膜作为对照组,进行免疫组化、垂直凝胶电泳、Western Blot、标志角蛋白衍生物刺激自体淋巴细胞增殖检测.结果:观察组样本CK阳性的例数明显高于对照组,且CK1、CK2、CK4、CK5、CK10、CK13、CK14、CK19存在显著差异,分离纯化的CK10、CK14能够明显促进T淋巴细胞的增殖.结论:CK10、CK14能够通过促进T淋巴细胞增殖和向病损区迁移来诱导扁平苔藓的发生.
목적:연구구강편평태선중불동류형각단백질적표체급기림상의의.방법:선택구강편평태선환자,선택편평태선표본작위관찰조、정상협점막작위대조조,진행면역조화、수직응효전영、Western Blot、표지각단백연생물자격자체림파세포증식검측.결과:관찰조양본CK양성적례수명현고우대조조,차CK1、CK2、CK4、CK5、CK10、CK13、CK14、CK19존재현저차이,분리순화적CK10、CK14능구명현촉진T림파세포적증식.결론:CK10、CK14능구통과촉진T림파세포증식화향병손구천이래유도편평태선적발생.
Objective:To study the expression and clinical significance of different types of keratin protein in oral lichen planus. Methods:Patients with oral lichen planus lichen were selected and divided into observation group and control group.Immunohistochemical, vertical gel electrophoresis, Western Blot, proliferation assay were conducted.Results:CK positive cases in the observation group sample was significantly higher than that in control group, and the CK1、CK2、CK4、CK5、CK10、CK13、CK14、CK19 were significantly different from each other. The separated and purificated CK can significantly promote the proliferation of T lymphocyte.Conclusion:CK10, CK14 can induce lichen planus occurring by promoting T lymphocytes proliferation and migration.