CAJ | 학술논문

目的以人的皮肤为材料,拟在体外成功分离培养成纤维前体细胞并研究其除皱整形能力. 方法组织块法分离人的皮肤成纤维前体细胞,并通过RT-PCR、流式细胞仪、软琼脂克隆等实验技术对分离的皮肤成纤维前体细胞进行鉴定,同时进行免疫荧光、动物实验检测其在治疗真皮损伤以及除皱整形方面的作用. 结果成功分离并扩增人的皮肤成纤维前体细胞,优化了原代细胞的分离方法,RT-PCR 和流式细胞仪检测结果显示该细胞表达部分干细胞表面标记 HLA-I、HLA-DR、CD90以及 CD105,软琼脂克隆实验表明该细胞不具有致瘤性.免疫荧光结果显示,注射该细胞的裸鼠与对照组相比,真皮层产生更多的 I 型胶原蛋白,皮肤厚度增加. 结论一次取材,体外能够成功持续获得人的原代皮肤成纤维前体细胞,优化了原代细胞分离方法.并且该细胞注射后可以在一定程度上分化为成纤维细胞,产生胶原蛋白,修复受损皮肤.
목적이인적피부위재료,의재체외성공분리배양성섬유전체세포병연구기제추정형능력. 방법조직괴법분리인적피부성섬유전체세포,병통과RT-PCR、류식세포의、연경지극륭등실험기술대분리적피부성섬유전체세포진행감정,동시진행면역형광、동물실험검측기재치료진피손상이급제추정형방면적작용. 결과성공분리병확증인적피부성섬유전체세포,우화료원대세포적분리방법,RT-PCR 화류식세포의검측결과현시해세포표체부분간세포표면표기 HLA-I、HLA-DR、CD90이급 CD105,연경지극륭실험표명해세포불구유치류성.면역형광결과현시,주사해세포적라서여대조조상비,진피층산생경다적 I 형효원단백,피부후도증가. 결론일차취재,체외능구성공지속획득인적원대피부성섬유전체세포,우화료원대세포분리방법.병차해세포주사후가이재일정정도상분화위성섬유세포,산생효원단백,수복수손피부.
Objective To isolate, culture and identify transit amplifying fibroblasts (TAFs) from human skin in vitro and detect its role in plastic surgery. @@@@Methods TAFs were isolated and cultured using tissue explants attachment method. The characteristics of TAFs were identified by RT-PCR, flow cytometry and soft agar. The collagen production of TAFs was detected by immunofluorescence in the animals. @@@@Results TAFs are successfully isolated and amplified. The TAFs express stem cell surface markers HLA-I HLA-DR, CD90 and CD105. The TAFs do not have the tumor formation capacity in the soft agar. Collagen I expression and skin thickness is increased in the nude mice group injected with TAFs as compared with control in the animal. @@@@Conclusion TAFs can be isolated and cultured in vitro, and can be injected in vivo to differentiate into fibroblasts and produce human collagen to cure the damaged skin.