中国医药生物技术
中國醫藥生物技術
중국의약생물기술
CHINESE MEDICINAL BIOTECHNOLOGY
2012年
6期
412-417
,共6页
申佳佳%苑隆国%李电东%王真
申佳佳%苑隆國%李電東%王真
신가가%원륭국%리전동%왕진
超氧化物歧化酶%过氧化脂质类%谷胱甘肽过氧化酶%衰老%红景天苷
超氧化物歧化酶%過氧化脂質類%穀胱甘肽過氧化酶%衰老%紅景天苷
초양화물기화매%과양화지질류%곡광감태과양화매%쇠로%홍경천감
Superoxide dismutase%Lipid peroxides%Glutathione peroxidase%Aging%Salidroside
目的通过检测红景天苷对自然衰老小鼠模型不同器官组织抗氧化酶活性及过氧化脂质水平的影响,探索红景天苷在老龄动物体内可能的抗衰老作用机制.
方法使用不同剂量(0.2 g/kg 和1.0 g/kg)的红景天苷给16月龄自然衰老小鼠灌胃60 d 后,检测小鼠血清、心脏、肝脏、肾脏和大脑等各器官组织匀浆中超氧化物歧化酶、谷胱甘肽过氧化酶的活性及过氧化脂质和脂褐素的水平.以人参皂苷为阳性药物.
结果与未加药对照组相比,红景天苷高剂量给药组自然衰老小鼠的血清、心脏、肝脏及肾脏的超氧化物歧化酶活性显著提高,而血清、肝脏、肾脏中过氧化脂质水平显著下降.该高剂量给药组自然衰老小鼠的心脏、肝脏、肾脏和大脑中脂褐素水平均较对照组降低,其中尤以大脑中的脂褐素水平降低最为显著.此外,高剂量红景天苷给药组小鼠的肾脏和大脑中谷胱甘肽过氧化酶活性与对照组相比出现一定程度的升高.
结论红景天苷能够有效提高自然衰老小鼠体内不同器官组织的抗氧化酶活性并且降低过氧化脂质和脂褐素水平,从而起到延缓衰老的作用.不同脏器组织对药物的反应性存在一定差异.
目的通過檢測紅景天苷對自然衰老小鼠模型不同器官組織抗氧化酶活性及過氧化脂質水平的影響,探索紅景天苷在老齡動物體內可能的抗衰老作用機製.
方法使用不同劑量(0.2 g/kg 和1.0 g/kg)的紅景天苷給16月齡自然衰老小鼠灌胃60 d 後,檢測小鼠血清、心髒、肝髒、腎髒和大腦等各器官組織勻漿中超氧化物歧化酶、穀胱甘肽過氧化酶的活性及過氧化脂質和脂褐素的水平.以人參皂苷為暘性藥物.
結果與未加藥對照組相比,紅景天苷高劑量給藥組自然衰老小鼠的血清、心髒、肝髒及腎髒的超氧化物歧化酶活性顯著提高,而血清、肝髒、腎髒中過氧化脂質水平顯著下降.該高劑量給藥組自然衰老小鼠的心髒、肝髒、腎髒和大腦中脂褐素水平均較對照組降低,其中尤以大腦中的脂褐素水平降低最為顯著.此外,高劑量紅景天苷給藥組小鼠的腎髒和大腦中穀胱甘肽過氧化酶活性與對照組相比齣現一定程度的升高.
結論紅景天苷能夠有效提高自然衰老小鼠體內不同器官組織的抗氧化酶活性併且降低過氧化脂質和脂褐素水平,從而起到延緩衰老的作用.不同髒器組織對藥物的反應性存在一定差異.
목적통과검측홍경천감대자연쇠로소서모형불동기관조직항양화매활성급과양화지질수평적영향,탐색홍경천감재노령동물체내가능적항쇠로작용궤제.
방법사용불동제량(0.2 g/kg 화1.0 g/kg)적홍경천감급16월령자연쇠로소서관위60 d 후,검측소서혈청、심장、간장、신장화대뇌등각기관조직균장중초양화물기화매、곡광감태과양화매적활성급과양화지질화지갈소적수평.이인삼조감위양성약물.
결과여미가약대조조상비,홍경천감고제량급약조자연쇠로소서적혈청、심장、간장급신장적초양화물기화매활성현저제고,이혈청、간장、신장중과양화지질수평현저하강.해고제량급약조자연쇠로소서적심장、간장、신장화대뇌중지갈소수평균교대조조강저,기중우이대뇌중적지갈소수평강저최위현저.차외,고제량홍경천감급약조소서적신장화대뇌중곡광감태과양화매활성여대조조상비출현일정정도적승고.
결론홍경천감능구유효제고자연쇠로소서체내불동기관조직적항양화매활성병차강저과양화지질화지갈소수평,종이기도연완쇠로적작용.불동장기조직대약물적반응성존재일정차이.
Objective To explore the possible anti-aging role of salidroside in naturally aged mice through testing the activity of antioxidant enzymes and the level of lipid peroxidation in some organs of the mice model.
@@@@Methods Different doses of salidroside (0.2 g/kg and 1.0 g/kg) were administrated to the naturally aged mice (16-month-old) for 60 days. The activity of superoxide dismutase (SOD) and GSH-Peroxidase (GSH-Px), as well as the level of Lipid peroxidation (LPO) and Lipofascin in multiple organs including plasma, heart, liver, kidney and brain of the mice was then detected and compared. Ginsenoside was used as a positive control.
@@@@Results SOD activity was significantly increased in the plasma, heart, liver and kidney of the mice group treated with high-dose salidroside, while the level of LPO was decreased in the plasma, liver and kidney of the same group, as compared with untreated control. The level of lipofascin was decreased in the heart, liver, kidney and brain of the high-dose mice group, with the level of change in brain being more significant as compared with untreated control. Moreover, GSH-Px activity was increased in the kidney and brain of the mice treated with high-dose salidroside, when compared with untreated control group.
@@@@Conclusion Salidroside plays an anti-aging role in vivo by increasing the activity of antioxidant enzymes and decreasing the level of lipid peroxidation in the naturally aged mice. The drug response may vary in different organs of the mice model.
