复乳法制备丝裂霉素C脂质体及包封率的测定
복유법제비사렬매소C지질체급포봉솔적측정
Determination of Entrapment Efficiency of Liposomal Mitomycin C by Double Emulsion
  • 万方数据
    中国医药指南 중국의약지남
    CHINA MEDICINE GUIDE
    2012年 34期 8-10 ,共3页

    杨冬发%覃健%侯之启*%罗琦%叶博材%何小杰%夏卫中%李沙%李日旺%陈璟昆%李延辉

    양동발%담건%후지계*%라기%협박재%하소걸%하위중%리사%리일왕%진경곤%리연휘

    丝裂霉素 C%脂质体%复乳法%包封率 絲裂黴素 C%脂質體%複乳法%包封率
    사렬매소 C%지질체%복유법%포봉솔
    Mitomycin C%Liposome%Double emulsification%Entrapment efficiency
    目的探讨复乳法制备丝裂霉素 C(MMC)脂质体的工艺方法和包封率测定.方法采用复乳法制备 MMC 脂质体,用透射电镜观察所制备的 MMC 脂质体的形态,选择 MMC 的吸收波长,葡聚糖凝胶柱分离游离 MMC 和 MMC 脂质体,测定脂质体混悬液中的总药物量与游离药物量,完成 MMC 脂质体包封率的测定.结果所制得的 MMC 脂质体呈球形与类球形,未过柱除去游离药物的 MMC 脂质体混悬液中 MMC 的浓度为341.16μg/mL,用柱层析分离除去游离药物后所得的 MMC 脂质体定容至一定体积,其中药物浓度为1.41μg/mL,MMC 脂质体平均包封率为8.25%.结论复乳法制得的脂质体的包封率较高,而且方法简单易行,药物产率也较高.
    목적탐토복유법제비사렬매소 C(MMC)지질체적공예방법화포봉솔측정.방법채용복유법제비 MMC 지질체,용투사전경관찰소제비적 MMC 지질체적형태,선택 MMC 적흡수파장,포취당응효주분리유리 MMC 화 MMC 지질체,측정지질체혼현액중적총약물량여유리약물량,완성 MMC 지질체포봉솔적측정.결과소제득적 MMC 지질체정구형여류구형,미과주제거유리약물적 MMC 지질체혼현액중 MMC 적농도위341.16μg/mL,용주층석분리제거유리약물후소득적 MMC 지질체정용지일정체적,기중약물농도위1.41μg/mL,MMC 지질체평균포봉솔위8.25%.결론복유법제득적지질체적포봉솔교고,이차방법간단역행,약물산솔야교고.
    Objective To study a method for determination of entrapment efficiency of liposomal Mitomycin C(MMC)by double emulsion. Methods Double emulsion was used to prepare liposomal MMC.The shape of liposomal MMC was observed under transmission electron microscope and the wavelength of MMC was chosen. MMC and liposomal MMC were separated by Glucose gelcolumn chromatography and the entrapment efficiency of liposomal MMC were determined. Results The shape of liposomal MMC was spherical. The concentration of MMC in liposomal MMC suspension which with free medicine was 341.16 μg/mL.After separating free medicine by Glucose gelcolumn chromatography, the concentration of liposomal MMC was 1.41μg/mL and the entrapment efficiency of liposomal MMC was 8.25%. Conclusion The entrapment efficiency of liposomal MMC by double emulsion is high. The method is simple and the productivity of medicine is high,too.
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