CAJ | 학술논문

目的建立愈肝片中测定维生素 C 的高效液相色谱法.方法采用 Agilent ZORBAX SB-Aq C18柱(250mm×4.6mm,5μm),柱温为25℃,以乙腈-0.1%的辛烷磺酸钠的0.1%的草酸溶液(2∶98)为流动相,流速1.0mL/min,检测波长为249nm.结果维生素 C 的线性范围为0.103~0.414μg(r=1.0000),平均回收率为99.3%,RSD 为1.3%.结论本方法简便,快速,准确,重现性好,可用于本品的质量控制.
목적건립유간편중측정유생소 C 적고효액상색보법.방법채용 Agilent ZORBAX SB-Aq C18주(250mm×4.6mm,5μm),주온위25℃,이을정-0.1%적신완광산납적0.1%적초산용액(2∶98)위류동상,류속1.0mL/min,검측파장위249nm.결과유생소 C 적선성범위위0.103~0.414μg(r=1.0000),평균회수솔위99.3%,RSD 위1.3%.결론본방법간편,쾌속,준학,중현성호,가용우본품적질량공제.
Objective To establish a method for the determination of Vitamin C in Yugan Tablets. Methods Agilent ZORBAX SB-Aq C18 column(4.6mm× 250mm, 5μm) was used as chromatographic column, the acetonitrile -0.1% octane sulfonate, 0.1% oxalic acid solution(2∶98 ) was used as mobile phase, the flow rate was 1.0mL/min. the detection wavelength was set at 249nm. Results The standard curves was linear in the rang of 0.103~0.414μg (r=-1.0000), the average recovery was 99.3%with a RSD of 1.3%. Conclusion The method is simple. accurate and reproducible, and is suitable for quality control of this tablets.