CAJ | 학술논문

目的建立以高效液相色谱法测定脉安颗粒中金丝桃苷含量的方法.方法色谱柱为 Kromasil C18柱,流动相为甲醇-0.2%磷酸溶液(37∶66),流速为1mL/min,检测波长为363nm.结果金丝桃苷浓度在2.008~20.06μg/mL 范围内的线性关系良好(r=0.9999),平均回收率为98.0%(RSD=1.23%).结论方法准确率高,重现性好,为本品的质量控制提供了可靠的方法.
목적건립이고효액상색보법측정맥안과립중금사도감함량적방법.방법색보주위 Kromasil C18주,류동상위갑순-0.2%린산용액(37∶66),류속위1mL/min,검측파장위363nm.결과금사도감농도재2.008~20.06μg/mL 범위내적선성관계량호(r=0.9999),평균회수솔위98.0%(RSD=1.23%).결론방법준학솔고,중현성호,위본품적질량공제제공료가고적방법.
Objective To establish a method for determining Hyperin in Mai’an Granules by HPLC. Methods Kromasil C18 chromatographic column with Methanol to 0.2% H3PO4 Water (37:66) as mobile phase. Current Velocity is at 1mL/min. WL was at 363nm for detection. Results The calibration curve was in good linear relation of Hyperin concentration over the range of 2.008~20.06μg/mL (r=0.9999). The average recovery was 98.0% (RSD=1.23%). Conclusion The method appears to be high in accuracy rate and with good in reproducibility. It offers a reliable method for controlling the quality of Mai’an Granules.