CAJ | 학술논문

目的探讨 PSA 值增高患者前列腺按摩后尿液沉渣中 DD3 mRNA 的表达,并讨论其临床意义.方法收集26例前列腺癌患者、50例前列腺增生症(BPH)患者前列腺按摩后尿液,离心取细胞沉淀物,提取总 RNA,采用逆转录-聚合酶链反应(RT-PCR)琼脂糖电泳法方法检测其中 DD3mRNA 的表达情况.结果前列腺癌患者前列腺按摩后尿液中 DD3 mRNA 阳性率明显高于前列腺增生患者,二者差异有统计学意义(P <0.01).不同 Gleason 分级研究组的前列腺癌患者 DD3 mRNA 表达阳性率无明显差别.结论患者 PSA 升高时,前列腺按摩后尿液中 DD3mRNA 检测是诊断前列腺癌的一种有效的方法,具有无侵入性,较前列腺穿刺活检的损伤更小.
목적탐토 PSA 치증고환자전렬선안마후뇨액침사중 DD3 mRNA 적표체,병토론기림상의의.방법수집26례전렬선암환자、50례전렬선증생증(BPH)환자전렬선안마후뇨액,리심취세포침정물,제취총 RNA,채용역전록-취합매련반응(RT-PCR)경지당전영법방법검측기중 DD3mRNA 적표체정황.결과전렬선암환자전렬선안마후뇨액중 DD3 mRNA 양성솔명현고우전렬선증생환자,이자차이유통계학의의(P <0.01).불동 Gleason 분급연구조적전렬선암환자 DD3 mRNA 표체양성솔무명현차별.결론환자 PSA 승고시,전렬선안마후뇨액중 DD3mRNA 검측시진단전렬선암적일충유효적방법,구유무침입성,교전렬선천자활검적손상경소.
Objective To study the expressions of differential display code 3 mRNA (DD3 mRNA) in urine sediments samples of patients with high PSA value and its diagnostic value. Methods The samples were collected from 50 benign prostatic hyperplasia and 26 prostate cancer patients before biopsy or operation, the total RNA had been extracted for RT-Polymerase chain reaction, agarose gel electrophoresis were applied to confirm PCR product. Results There was significant difference of DD3 mRNA positive rate in urine sediments obtained after prostatic massage between BPH and PCa patients (P<0.01). DD3mRNA positive rate has no significant difference between Gleason grade.Conclusion Detection of DD3mRNA in urine sediments obtained after prostatic massage has excellent diagnose efficacy for prostate cancer as a promising and independent non-invasive method.