中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
2期
401-402
,共2页
止咳枇杷糖浆%齐墩果酸%熊果酸%高效液相色谱
止咳枇杷糖漿%齊墩果痠%熊果痠%高效液相色譜
지해비파당장%제돈과산%웅과산%고효액상색보
Zhike Pipa Tangjiang%Oleanolic acid%Ursolic acid%HPLC
目的建立止咳枇杷糖浆中齐墩果酸及熊果酸的含量测定方法.方法采用Apollo C18色谱柱(4.6mm×250mm,5mm),以乙腈-甲醇-0.5%醋酸铵溶液(67∶12∶21)为流动相,流速1.0mL/min,检测波长为210nm.结果齐墩果酸含量在0.1006~1.5090μg范围内呈良好的线性关系,r=0.9999,平均回收率为98.73%,RSD为1.05%;熊果酸含量在0.4028~6.0420μg范围内呈良好的线性关系, r=0.9997,平均回收率为99.25%,RSD为1.54%.结论该方法简便、结果准确、重现性好,可用于止咳枇杷糖浆的质量控制.
目的建立止咳枇杷糖漿中齊墩果痠及熊果痠的含量測定方法.方法採用Apollo C18色譜柱(4.6mm×250mm,5mm),以乙腈-甲醇-0.5%醋痠銨溶液(67∶12∶21)為流動相,流速1.0mL/min,檢測波長為210nm.結果齊墩果痠含量在0.1006~1.5090μg範圍內呈良好的線性關繫,r=0.9999,平均迴收率為98.73%,RSD為1.05%;熊果痠含量在0.4028~6.0420μg範圍內呈良好的線性關繫, r=0.9997,平均迴收率為99.25%,RSD為1.54%.結論該方法簡便、結果準確、重現性好,可用于止咳枇杷糖漿的質量控製.
목적건립지해비파당장중제돈과산급웅과산적함량측정방법.방법채용Apollo C18색보주(4.6mm×250mm,5mm),이을정-갑순-0.5%작산안용액(67∶12∶21)위류동상,류속1.0mL/min,검측파장위210nm.결과제돈과산함량재0.1006~1.5090μg범위내정량호적선성관계,r=0.9999,평균회수솔위98.73%,RSD위1.05%;웅과산함량재0.4028~6.0420μg범위내정량호적선성관계, r=0.9997,평균회수솔위99.25%,RSD위1.54%.결론해방법간편、결과준학、중현성호,가용우지해비파당장적질량공제.
Objective To establish the determination method of Oleanolic acid and Ursolic in Zhike Pipa Tangjiang by HPLC. Methods The separation was out on a Apollo C18 column , with mobile phase of acetonitrile-methanol-0.5%ammonium acetate(67∶12∶21). The flow rale was set at 1.0mL/min and the detection wavelength was at 210nm. Result The calibration curve of oleanolic acid was in good linear of 0.1006-1.5090μg, (r=0.9999), and the average recovery rate was 98.73%, RSD=1.05%. Ursolic showed good linear of 0.4028-6.0420μg, (r=0.9997), and the average recovery rate was 99.25%, RSD=1.54%. Conclusion The developed method is sensitive, accurate, and with a good reproducibility, and can be used for the quality control of the granules.
