CAJ | 학술논문

目的:研究间皮细胞对胃癌细胞的抵御作用,模拟游离癌细胞在接触腹膜前通过其分泌物导致的腹膜增厚、纤维化的过程,同时观察了间皮细胞对胃癌细胞迁移及侵袭能力的反作用.方法:用荧光显微镜观察共培养时间皮细胞对胃癌细胞的抵御作用;体内实验观察腹膜变化;电镜、光镜下观察体外实验中间皮层在胃癌-腹膜相互作用中的损伤和细胞骨架变化;采用Mil?licell小室共培养观察间皮细胞对胃癌细胞迁移及侵袭能力的反作用.结果:正常间皮细胞可防止肿瘤细胞对于腹膜的粘附,受损脱落后胃癌细胞可轻易粘附.体内外实验均显示接触胃癌细胞上清后腹膜间皮细胞受损、凋亡,并且受损残余的间皮可以反作用于癌细胞,使其迁移转移力提高.结论:正常间皮细胞可以抵御胃癌细胞的侵袭,受损伤刺激后的间皮细胞可以反作用于胃癌细胞促进其迁移及侵袭.
목적:연구간피세포대위암세포적저어작용,모의유리암세포재접촉복막전통과기분비물도치적복막증후、섬유화적과정,동시관찰료간피세포대위암세포천이급침습능력적반작용.방법:용형광현미경관찰공배양시간피세포대위암세포적저어작용;체내실험관찰복막변화;전경、광경하관찰체외실험중간피층재위암-복막상호작용중적손상화세포골가변화;채용Mil?licell소실공배양관찰간피세포대위암세포천이급침습능력적반작용.결과:정상간피세포가방지종류세포대우복막적점부,수손탈락후위암세포가경역점부.체내외실험균현시접촉위암세포상청후복막간피세포수손、조망,병차수손잔여적간피가이반작용우암세포,사기천이전이력제고.결론:정상간피세포가이저어위암세포적침습,수손상자격후적간피세포가이반작용우위암세포촉진기천이급침습.
Objective: This study aims to describe the mechanism by which gastric cancer cells lead to an early peritoneal metas-tasis. Methods: Injured mesothelial cells were examined by fluorescence microscopy. A rat's parietal thickness was measured using he-matoxylin and eosin staining. Morphological changes in the human peritoneal mesothelial cells (HPMC) were observed under a scan-ning electron microscope and phase-contrast microscope. The cell migration capacity and the gastric cancer cell infestation were exam-ined using Millicell inserts in a chamber co-culture. Results: Mesothelial cells could prevent cancer cell infiltration into the sub-meso-thelial connective tissues. Conspicuous morphological changes were observed in the HPMC after the treatment with the gastric cancer cell supernatants. The supernatants induced the cytoskeleton reconstruction of HPMC. The injured mesothelial cells promoted invasion and metastasis in the gastric cancer cells. Conclusion:These findings indicate that gastric cancer cells can induce peritoneal fibrosis through supernatants during early peritoneal metastasis. However, the injured mesothelial cells can upregulate invasion and metastasis in gastric cancer cells.