CAJ | 학술논문

目的:探讨微小RNA-150(miR-150)在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血单个核细胞(pe?ripheral blood mononuclear cells,PBMC)中的表达及其临床意义.方法:采用实时定量PCR(quantitative reverse-transcriptase PCR, RT-qPCR)检测64例NSCLC患者、26例正常对照患者PBMC中miR-150表达量,并且分析其对NSCLC的诊断价值及与NSCLC临床病理参数间的关系.结果:miR-150水平在NSCLC组和对照组间差异无统计学意义(P=0.260),但是肺腺癌组miR-150水平显著高于对照组(P=0.001)、肺鳞癌组(P<0.001).ROC曲线分析miR-150对NSCLC诊断的AUC为0.610(95%CI:0.493~0.728,P=0.060),miR-150对肺腺癌诊断的AUC为0.834(95%CI:0.734~0.934,P<0.01),而miR-150对肺腺癌和肺鳞癌鉴别诊断的AUC为0.951(95%CI:0.903~0.999,P<0.01).NSCLC 患者 miR-150水平与肿瘤远处转移密切相关(P=0.014),与临床分期、淋巴结转移无关(P>0.05).结论:肺腺癌患者PBMC中miR-150表达明显升高,并且可能成为鉴别诊断肺腺癌和肺鳞癌的新标志物.
목적:탐토미소RNA-150(miR-150)재비소세포폐암(non-small cell lung cancer,NSCLC)환자외주혈단개핵세포(pe?ripheral blood mononuclear cells,PBMC)중적표체급기림상의의.방법:채용실시정량PCR(quantitative reverse-transcriptase PCR, RT-qPCR)검측64례NSCLC환자、26례정상대조환자PBMC중miR-150표체량,병차분석기대NSCLC적진단개치급여NSCLC림상병리삼수간적관계.결과:miR-150수평재NSCLC조화대조조간차이무통계학의의(P=0.260),단시폐선암조miR-150수평현저고우대조조(P=0.001)、폐린암조(P<0.001).ROC곡선분석miR-150대NSCLC진단적AUC위0.610(95%CI:0.493~0.728,P=0.060),miR-150대폐선암진단적AUC위0.834(95%CI:0.734~0.934,P<0.01),이miR-150대폐선암화폐린암감별진단적AUC위0.951(95%CI:0.903~0.999,P<0.01).NSCLC 환자 miR-150수평여종류원처전이밀절상관(P=0.014),여림상분기、림파결전이무관(P>0.05).결론:폐선암환자PBMC중miR-150표체명현승고,병차가능성위감별진단폐선암화폐린암적신표지물.
Objective: To investigate the expression and clinical significance of miR-150 in non-small cell lung cancer (NSCLC). Methods: Probe-based stem-loop quantitative reverse-transcriptase polymerase chain reaction was used to detect the miR-150 expression in peripheral blood mononuclear cells (PBMC) of 64 patients with NSCLC and 26 healthy individuals, and the relationship between miR-150 level and clinicopathological factors was explored. Results: miR-150 expressions in PBMC specimens between the cancer patients and the healthy individuals was not significantly different (P=0.260). The miR-150 expression in lung adenocarcinoma patients was significantly higher than that in healthy individuals (P=0.001). miR-150 expressions between the adenocarcinoma and squamous cells in NSCLC showed very strong differences (P=0.000). miR-150 expressions showed low discriminative ROC curve profiles, distinguishing NSCLC patients from healthy subjects by the area under the ROC curve ( AUC ) at 0.610 ( 95% CI 0.493 to 0.728 ) (P =0.060). High expression of miR-150 was identified as a moderately specific marker for lung adenocarcinoma, distinguishing patients from healthy subjects with AUC at 0.834 ( 95 % CI 0.734 to 0.934 ) (P=0.000). Additionally, miR-150 was identified as a highly specific marker for adenocarcinoma distinguished from squamous cell carcinoma in NSCLC with AUC at 0.951 ( 95% CI 0.903 to 0.999 ) (P=0.000). The miR-150 level was significantly associated with distant metastasis (P=0.014). No association was found between miR-150 and other clinicopathological features, such as clinical stage and lymphatic metastasis. Conclusion: miR-150 expression in PBMC specimens is significantly increased in lung adeno-carcinoma patients. miR-150 can be a highly accurate marker for differentiating adenocarcinoma from squamous cell carcinoma in NSCLC.