中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
1期
21-24
,共4页
宋世铎%周健%何宋兵%朱东明%赵华%张子祥%王毅%李德春
宋世鐸%週健%何宋兵%硃東明%趙華%張子祥%王毅%李德春
송세탁%주건%하송병%주동명%조화%장자상%왕의%리덕춘
胰腺癌%miRNA-375%增殖%凋亡
胰腺癌%miRNA-375%增殖%凋亡
이선암%miRNA-375%증식%조망
pancreatic cancer%miRNA-375%proliferation%apoptosis
目的:探讨miRNA-375的外源性表达对人胰腺癌细胞Panc-1增殖和凋亡的影响.方法:将miRNA-375表达质粒或对照质粒转染Panc-1细胞,qRT-PCR方法检测miRNA-375在转染细胞中的表达水平,细胞计数法、流式细胞术分别检测外源性miRNA-375表达后Panc-1细胞增殖、凋亡和周期的变化情况.结果:miRNA-375表达质粒组与对照组比较,其miRNA-375表达诱导性升高,细胞增殖受到抑制,细胞凋亡率增加,细胞周期阻滞于G0~G1期.结论:在胰腺癌细胞Panc-1中增加miRNA-375的表达,可抑制细胞增殖,促进细胞凋亡和细胞周期阻滞,miRNA-375在胰腺癌细胞Panc-1中可能发挥潜在的抑癌基因作用.
目的:探討miRNA-375的外源性錶達對人胰腺癌細胞Panc-1增殖和凋亡的影響.方法:將miRNA-375錶達質粒或對照質粒轉染Panc-1細胞,qRT-PCR方法檢測miRNA-375在轉染細胞中的錶達水平,細胞計數法、流式細胞術分彆檢測外源性miRNA-375錶達後Panc-1細胞增殖、凋亡和週期的變化情況.結果:miRNA-375錶達質粒組與對照組比較,其miRNA-375錶達誘導性升高,細胞增殖受到抑製,細胞凋亡率增加,細胞週期阻滯于G0~G1期.結論:在胰腺癌細胞Panc-1中增加miRNA-375的錶達,可抑製細胞增殖,促進細胞凋亡和細胞週期阻滯,miRNA-375在胰腺癌細胞Panc-1中可能髮揮潛在的抑癌基因作用.
목적:탐토miRNA-375적외원성표체대인이선암세포Panc-1증식화조망적영향.방법:장miRNA-375표체질립혹대조질립전염Panc-1세포,qRT-PCR방법검측miRNA-375재전염세포중적표체수평,세포계수법、류식세포술분별검측외원성miRNA-375표체후Panc-1세포증식、조망화주기적변화정황.결과:miRNA-375표체질립조여대조조비교,기miRNA-375표체유도성승고,세포증식수도억제,세포조망솔증가,세포주기조체우G0~G1기.결론:재이선암세포Panc-1중증가miRNA-375적표체,가억제세포증식,촉진세포조망화세포주기조체,miRNA-375재이선암세포Panc-1중가능발휘잠재적억암기인작용.
Objective: This study aimed to investigate the effect of the ectogenesis expression of miRNA-375 on cell proliferation and apoptosis in the human pancreatic cancer cell line Panc-1. Methods: After transfection of the miRNA-375 vector, the expression levels of miRNA-375 in the Panc-1 cells were determined by quantitative real-time polymerase chain reaction. Cell counting was performed to evaluate the effects of miRNA-375 expression on cell proliferation. Flow cytometry was used to analyze apoptosis and the cell cycle. Results:The expression levels of miRNA-375 were highly induced after miRNA-375 transfection. miRNA-375 restoration also inhibited cell proliferation, promoted cell apoptosis, and induced cell cycle arrest at G0-G1. Conclusion: The abnormal expression of miRNA-375 in the pancreatic cancer cell line Panc-1 inhibits the canceration of cells by interfering with cellular biological behaviors, such as proliferation and apoptosis. miRNA-375 may act as a potential tumor suppressor in the pancreatic cancer cell Panc-1.