CAJ | 학술논문

目的:探讨血清高尔基体蛋白73(GP73)及其与甲胎蛋白(AFP)和CA199联合检测对原发性肝癌(PHC)诊断的价值.方法:收集肝癌患者50例、肝炎患者20例、肝硬化患者20例、除肝癌外其他消化系统恶性肿瘤患者20例及健康对照者20例.用酶联免疫分析方法定量检测各组人群血清GP73,用电化学发光法测定AFP和CA199.结果:肝癌组血清GP73含量中位数(104.4μg/L)明显高于肝炎组(22.7μg/L)、肝硬化组(53.8μg/L)、其他消化系统肿瘤组(41.4μg/L)和对照组(19.3μg/L),P均<0.05.单项指标中GP73用于肝癌诊断的灵敏度、特异性和准确率均最高,分别为72.0%、95.0%和86.2%,GP73/AFP/CA199平行实验灵敏度最高(92.0%), GP73+AFP+CA199系列实验特异性最高(100%),GP73/AFP平行实验准确率最高(87.7%).ROC曲线分析GP73曲线下面积最大,为0.824.三个检测指标之间相关性分析无统计学意义(P>0.05).结论:GP73是一种新的更有效的肝癌诊断的血清标志物,其联合AFP和CA199检测可提高对PHC的诊断价值.
목적:탐토혈청고이기체단백73(GP73)급기여갑태단백(AFP)화CA199연합검측대원발성간암(PHC)진단적개치.방법:수집간암환자50례、간염환자20례、간경화환자20례、제간암외기타소화계통악성종류환자20례급건강대조자20례.용매련면역분석방법정량검측각조인군혈청GP73,용전화학발광법측정AFP화CA199.결과:간암조혈청GP73함량중위수(104.4μg/L)명현고우간염조(22.7μg/L)、간경화조(53.8μg/L)、기타소화계통종류조(41.4μg/L)화대조조(19.3μg/L),P균<0.05.단항지표중GP73용우간암진단적령민도、특이성화준학솔균최고,분별위72.0%、95.0%화86.2%,GP73/AFP/CA199평행실험령민도최고(92.0%), GP73+AFP+CA199계렬실험특이성최고(100%),GP73/AFP평행실험준학솔최고(87.7%).ROC곡선분석GP73곡선하면적최대,위0.824.삼개검측지표지간상관성분석무통계학의의(P>0.05).결론:GP73시일충신적경유효적간암진단적혈청표지물,기연합AFP화CA199검측가제고대PHC적진단개치.
Objective: To investigate the clinical value of serum Glogi glycoprotein 73 (GP73) and its combination to alpha-fetoprotein (AFP) and CA199 for the diagnosis of primary hepatic cancer (PHC). Methods: Sera from 110 patients (50 hepatic cancer, 20 hepatitis, 20 liver cirrhosis, and 20 malignant tumors of the digestive system except hepatic cancer) and 20 healthy controls were collected. Serum GP73 was analyzed by enzyme-1inked immunosorbent assay. The levels of AFP and CA199 were quantified by electrochemiluminescence im-munoassays. Results: The median serum GP73 concentration of PHC (104.4 μg/L) was higher than that of hepatitis (22.7 μg/L), liver cirrhosis (53.8 μg/L), malignant tumors of the digestive system except hepatic cancer (41.4 μg/L), and healthy controls (19.3 μg/L) significantly (P<0.05). The sensitivity, specificity, and accuracy to diagnose PHC by GP73 alone were 72.0%, 95.0% and 86.2%, respectively, and were higher than that of AFP or CA199 alone. GP73's sensitivity was highest (92.0%) for the parallel test of GP73, AFP, and CA199; its specificity was highest (100.0%) for the series-wound test of GP73, AFP, and CA199; and its accuracy was the highest (87.7%) for the parallel test of GP73 and AFP. The area under the ROC curve of GP73 was 0.824, which is higher than that of AFP or CA199 alone. The serum GP73 level was not significantly correlated with that of AFP or CA199. Conclusion: GP73 is a new effective marker in the diagnosis of PHC, and the combined examination of GP73, AFP, and CA199 can improve the diagnosis value of PHC.