CAJ | 학술논문

目的:探讨胃癌组织中miRNA-375基因表达与基因甲基化调控的相关性.方法:2011年3月至8月在天津医科大学总医院通过胃镜检查收集90例新鲜组织活检标本,分为2组,胃癌组54例,非癌对照组36例.应用实时荧光定量反转录PCR检测miRNA-375基因表达,甲基化特异性PCR检测miRNA-375基因启动子区CpG岛甲基化.结果:胃癌组miRNA-375基因表达下调,与非癌对照组相比差异有统计学意义(P<0.05);胃癌组和非癌对照组 miRNA-375基因启动子区高甲基化阳性率分别为62.96%(34/54)和22.22%(8/36),差异有统计学意义(χ2=14.405, P<0.05).中高分化胃癌组织中miRNA-375基因表达高于低分化组,差异有统计学意义(t=2.634,P=0.011);miRNA-375基因启动子区甲基化阳性率中高分化组与低分化组分别为44.44%(8/18)和72.22%(26/36),差异有统计学意义(χ2=3.971,P=0.046).结论:癌组织中存在miRNA-375基因异常低表达及启动子区的高甲基化,miRNA-375基因高甲基化可能抑制miRNA-375基因表达,在胃癌发生发展中发挥重要作用.
목적:탐토위암조직중miRNA-375기인표체여기인갑기화조공적상관성.방법:2011년3월지8월재천진의과대학총의원통과위경검사수집90례신선조직활검표본,분위2조,위암조54례,비암대조조36례.응용실시형광정량반전록PCR검측miRNA-375기인표체,갑기화특이성PCR검측miRNA-375기인계동자구CpG도갑기화.결과:위암조miRNA-375기인표체하조,여비암대조조상비차이유통계학의의(P<0.05);위암조화비암대조조 miRNA-375기인계동자구고갑기화양성솔분별위62.96%(34/54)화22.22%(8/36),차이유통계학의의(χ2=14.405, P<0.05).중고분화위암조직중miRNA-375기인표체고우저분화조,차이유통계학의의(t=2.634,P=0.011);miRNA-375기인계동자구갑기화양성솔중고분화조여저분화조분별위44.44%(8/18)화72.22%(26/36),차이유통계학의의(χ2=3.971,P=0.046).결론:암조직중존재miRNA-375기인이상저표체급계동자구적고갑기화,miRNA-375기인고갑기화가능억제miRNA-375기인표체,재위암발생발전중발휘중요작용.
Objective: This study aimed to explore aberrant DNA methylation of microRNA-375 (miRNA-375) gene and its expression in gastric carcinoma tissues. Methods: A total of 90 subjects were divided into two groups: gastric carcinoma (n=54) and non-cancer control (n = 36). The expression of miRNA-375 gene was detected by real-time fluorescent quantitative reverse-transcription polymerase chain reaction. The DNA methylation of the CpG island promoters of miRNA-375 was detected by the DNA methylation specific polymerase chain reaction in gastric carcinoma and non-cancer control mucosa. Results: The expression of miRNA-375 in the gastric carcinoma significantly decreased compared with the non-cancer control (P=0.000, P<0.05). The positive rate of the hypermethylation of miRNA-375 gene (62.96%) in the gastric carcinoma was significantly higher than that in the non-cancer control (22.22%) (χ2=14.405, P=0.000, P<0.05). The expression of miRNA-375 gene in well-differentiated carcinoma was significantly higher than that in poorly differentiated carcinoma. A statistically significant difference was found between the two groups (t=2.634, P=0.011, P<0.05). The positive rate of DNA hypermethylation of miRNA-375 gene (44.44%, 8/18) in well-differentiated carcinoma was significantly higher than that in poorly differentiated carcinoma (72.22%, 26/36) (χ2=3.971, P=0.046, P<0.05). Conclusion: The aberrant hypermethylation of the CpG island promoters of miRNA-375 gene and their lower expression in gastric carcinoma may play a crucial role in carcinogenesis and gastric carcinoma development.