CAJ | 학술논문

目的:探讨新型组蛋白去乙酰化酶抑制剂LBH589对人上皮性卵巢癌OVCAR-3细胞增殖抑制和促进凋亡作用及其机制.方法:不同浓度LBH589处理细胞后,采用噻唑蓝(MTT)比色法检测对细胞增殖的影响;AO/EB(台盼蓝、吖啶橙溴化乙啶双染色法)检测细胞凋亡;Western blot 检测聚腺苷二磷酸核糖聚合酶(PARP)、Caspase-3、Bcl-2、Bax蛋白水平.结果:LBH589明显抑制OVCAR-3细胞增殖,48 h半数抑制浓度(IC50)为0.12μM.Western blot检测发现Caspsae-3、PARP-85kD剪切蛋白增加,Bax表达增加,Bcl-2表达减少.结论:LBH589在体外条件下能明显抑制卵巢癌细胞OVCAR-3细胞增殖,诱导细胞凋亡.
목적:탐토신형조단백거을선화매억제제LBH589대인상피성란소암OVCAR-3세포증식억제화촉진조망작용급기궤제.방법:불동농도LBH589처리세포후,채용새서람(MTT)비색법검측대세포증식적영향;AO/EB(태반람、아정등추화을정쌍염색법)검측세포조망;Western blot 검측취선감이린산핵당취합매(PARP)、Caspase-3、Bcl-2、Bax단백수평.결과:LBH589명현억제OVCAR-3세포증식,48 h반수억제농도(IC50)위0.12μM.Western blot검측발현Caspsae-3、PARP-85kD전절단백증가,Bax표체증가,Bcl-2표체감소.결론:LBH589재체외조건하능명현억제란소암세포OVCAR-3세포증식,유도세포조망.
Objective: This study was designed to investigate the mechanism and effect of LBH589, a novel histone deacetylase inhibitor, on the growth and apoptosis of the human ovarian cancer cell line OVCAR-3. Methods: The cells were treated with LBH589 at different concentrations. Cell proliferation was determined using the methyl thiazol tetrazolium assay. The apoptotic rate of the cells was detected by AO/EB double-staining. Protein expressions of polyADP-ribose polymerase (PARP), caspase-3, bel-2, and bax were analyzed by western blot assay. Results: LBH589 significantly inhibited the proliferation of OVCAR-3 cells at a concentration of 0.12 μM/L based on a 48 h half-inhibitory concentration (IC50). Western blot assay showed that the expressions of cleaved PARP-85KD, caspase-3, and bax were increased, but bcl-2 expression was downregulated. Conclusion: LBH589 in vitro can significantly inhibit the proliferation and induce apoptosis of the human ovarian cancer cell line OVCAR-3.