CAJ | 학술논문

目的:探讨 E-钙黏蛋白(E-cadherin,E-cad)、P120连环蛋白(P120ctn)在乳腺浸润性导管癌(IDC)和浸润性小叶癌(ILC)中的表达及其鉴别诊断意义.方法:收集解放军第113医院2007年12月至2012年5月间有完整随访资料的浸润性导管癌60例、浸润性小叶癌48例及混合性癌20例.采用免疫组织化学S-P法检测E-cad及P120的表达.结果:E-cad在IDC和ILC中的阳性率分别为85%(51/60)和0,其差异有统计学意义(P<0.01);P120在IDC中阳性率为100%,且均为细胞膜着色,而在ILC中阳性率亦为100%,但均为胞浆着色.E-cad和P120联合使用将20例混合癌确诊为IDC 16例及ILC 4例.IDC中E-cad的表达水平与肿瘤分期无关,与淋巴结转移有关(P<0.05).结论:E-cad和P120联合使用可以鉴别光镜下易混淆的IDC和ILC,使组织分型更准确,建议应用于乳腺癌的常规免疫组织化学检测.E-cad的表达水平与淋巴结转移有关.
목적:탐토 E-개점단백(E-cadherin,E-cad)、P120련배단백(P120ctn)재유선침윤성도관암(IDC)화침윤성소협암(ILC)중적표체급기감별진단의의.방법:수집해방군제113의원2007년12월지2012년5월간유완정수방자료적침윤성도관암60례、침윤성소협암48례급혼합성암20례.채용면역조직화학S-P법검측E-cad급P120적표체.결과:E-cad재IDC화ILC중적양성솔분별위85%(51/60)화0,기차이유통계학의의(P<0.01);P120재IDC중양성솔위100%,차균위세포막착색,이재ILC중양성솔역위100%,단균위포장착색.E-cad화P120연합사용장20례혼합암학진위IDC 16례급ILC 4례.IDC중E-cad적표체수평여종류분기무관,여림파결전이유관(P<0.05).결론:E-cad화P120연합사용가이감별광경하역혼효적IDC화ILC,사조직분형경준학,건의응용우유선암적상규면역조직화학검측.E-cad적표체수평여림파결전이유관.
Objective: To explore the expression of E-cadherin and p120 catenin in invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) and their significance for differential diagnosis. Methods: A total of 60 cases with IDCs, 48 invasive lobular carcinomas, and 20 invasive carcinomas with mixed ductal and lobular features were collected from No. 113 Hospital of People's Liberation Army. An immunohistochemical study for E-cadherin and P120 catenin was performed using a streptavidin–peroxidase method. Results:The positivity rates for E-cadherin in IDC and classic ILC were 85% (51/60) and 0%, respectively (P<0.01). The positivity rates for p120 catenin were 100% in both IDC (membranous staining) and classic ILC (cytoplasmic staining). E-cadherin and p120 catenin were used to classify 20 mixed carcinomas into 16 IDCs and 4 ILCs. No significant difference was found in E-cadherin expression level in different histological types of IDC was indicated. However, E-cadherin expression was coincided with lymph node metastasis (P<0.05). Conclusion:E-cadherin and p120 catenin are useful immunomarkers to distinguish IDCs from ILCs and thus can be used in routine immunohistochemistry in breast cancer. The E-cad expression level coincides with lymph node metastasis in IDC.