CAJ | 학술논문

目的:探讨分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)、白细胞介素-6(interleukin-6,IL-6)和树突状细胞(dendritic cell,DC)在口腔癌中的表达和相互作用.方法:选取60例原发口腔癌患者为研究对象,20例健康志愿者唾液为正常对照,用ELISA法检测唾液中的SIgA、IL-6含量.用免疫组织化学法和流式细胞仪检测癌组织中CD1a、CD83、CD80及CD86的表达情况.经病理证实的20例良性肿瘤患者正常口腔黏膜组织为对照.结果:口腔癌患者唾液SIgA的含量明显低于对照组,IL-6的含量明显高于对照组,差异有统计学意义(P<0.05),二者之间呈负相关(r=-0.993,P<0.05).CD1a、CD83、CD80、CD86在癌组织中的表达低于对照组织(P<0.05).CD80、CD86的表达与组织学分级、病理类型无关(P>0.05),与临床分期及淋巴结转移呈负相关(P<0.05).结论:唾液SIgA和IL-6含量可以作为口腔癌的辅助诊断指标,IL-6的升高可能是导致SIgA减少的原因之一.口腔癌组织中DC存在免疫缺陷,对DC表面CD80、CD86的检测有助于评判预后.IL-6可能通过抑制SIgA的生成,导致DC免疫耐受,无法激活有效免疫应答,从而促进口腔癌的发生发展.
목적:탐토분비형면역구단백A(secretory immunoglobulin A,SIgA)、백세포개소-6(interleukin-6,IL-6)화수돌상세포(dendritic cell,DC)재구강암중적표체화상호작용.방법:선취60례원발구강암환자위연구대상,20례건강지원자타액위정상대조,용ELISA법검측타액중적SIgA、IL-6함량.용면역조직화학법화류식세포의검측암조직중CD1a、CD83、CD80급CD86적표체정황.경병리증실적20례량성종류환자정상구강점막조직위대조.결과:구강암환자타액SIgA적함량명현저우대조조,IL-6적함량명현고우대조조,차이유통계학의의(P<0.05),이자지간정부상관(r=-0.993,P<0.05).CD1a、CD83、CD80、CD86재암조직중적표체저우대조조직(P<0.05).CD80、CD86적표체여조직학분급、병리류형무관(P>0.05),여림상분기급림파결전이정부상관(P<0.05).결론:타액SIgA화IL-6함량가이작위구강암적보조진단지표,IL-6적승고가능시도치SIgA감소적원인지일.구강암조직중DC존재면역결함,대DC표면CD80、CD86적검측유조우평판예후.IL-6가능통과억제SIgA적생성,도치DC면역내수,무법격활유효면역응답,종이촉진구강암적발생발전.
Objective:This work studied the interaction of secretory immunoglobulin A (SIgA), interleukin 6 (IL-6), and dendritic cells (DCs) in oral cancer. Methods:Saliva samples from each oral cancer patient were collected before surgery to detect the SIgA and IL-6 levels. Enzyme-linked immunosorbent assay and flow cytometry were used to determine the expression of CD1a, CD83, CD80, and CD86 on DCs in cancer tissues. Normal oral epithelial tissues were confirmed by pathology and used as the controls. Results:The SIgA content was lower in the saliva of oral cancer patients than in those of the normal individuals. By contrast, the IL-6 content was evidently higher in the saliva of cancer patients than in that of the healthy controls. SIgA and IL-6 were negatively correlated with each other (r=-0.993, P<0.05). The expression levels of CD1a, CD83, CD80, and CD86 in cancer tissues were lower than those in normal tissues (P<0.05). The CD80 and CD86 expression levels in the cancer tissues were not associated with the histological grading and pathological types (P>0.05). Correlation analysis showed that the CD80 and CD86 levels were negatively correlated with the clinical stage and lymph node metastases (P<0.05). Conclusion:The SIgA and IL-6 content can be used as auxiliary indicators for oral cancer diagnosis. The increasing IL-6 level could account for the decreased SIgA production. Immune deficiency occurs in the DCs of oral cancer patients, and the expression levels of CD80 and CD86 reflect this prognostic evaluation. IL-6 may inhibit the formation of SIgA and cause the immune tolerance of DC. Thus, the effective immune response is lost, thereby promoting carcinogenesis and the progress of oral cancer.