中国中西医结合急救杂志
中國中西醫結閤急救雜誌
중국중서의결합급구잡지
INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE IN PRACTICE OF CRITICAL CARE MEDICINE
2012年
6期
343-347
,共5页
卢晗%葛赟%洪广亮%姜小珍%刘瑶%邱俏檬%卢中秋
盧晗%葛赟%洪廣亮%薑小珍%劉瑤%邱俏檬%盧中鞦
로함%갈빈%홍엄량%강소진%류요%구초몽%로중추
硫化氢%血必净注射液%肺损伤,急性%核转录因子-κB%肿瘤坏死因子-α%白细胞介素
硫化氫%血必淨註射液%肺損傷,急性%覈轉錄因子-κB%腫瘤壞死因子-α%白細胞介素
류화경%혈필정주사액%폐손상,급성%핵전록인자-κB%종류배사인자-α%백세포개소
Hydrogen sulfide%Xuebijing injection%Acute lung injury%Nuclear factor-κB%Tumor necrosis factor-α%Interleukin
目的探讨血必净注射液对硫化氢(H2S)急性中毒大鼠肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL-6、IL-10)及核转录因子-κB(NF-κB)的影响.方法将96只清洁级 SD 大鼠按随机数字表法分为正常对照组(n =8)、血必净对照组(n =8)、H2S 染毒模型组(n =40)和血必净干预组(n =40).采用酶联免疫吸附试验(ELISA)测定肺组织中 TNF-α、IL-6、IL-10水平;用逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blotting)检测肺组织 NF-κB mRNA 和蛋白表达;光镜下观察肺组织病理学改变.结果正常对照组和血必净对照组 TNF-α、IL-6、IL-10水平及 NF-κB mRNA 和蛋白表达比较差异均无统计学意义(均P>0.05).与正常对照组比较,H2S 染毒模型组在染毒后2、6、12、24、48 h TNF-α(ng/L)、IL-6(ng/L)、IL-10(ng/L)水平及 NF-κB mRNA 和蛋白表达明显升高(均P<0.01),分别于12、48、48、24、24 h 达峰值(TNF-α12 h:400.79±13.74,IL-648 h :242.69±8.96,IL-1048 h :353.73±10.75,NF-κB mRNA 24 h :0.568±0.007,NF-κB蛋白24 h :0.520±0.007);与 H2S 染毒模型组同期比较,血必净干预组 TNF-α、IL-6水平及 NF-κB mRNA和蛋白于2 h 开始即明显降低(TNF-α:322.46±10.16比377.89±10.87,IL-6:124.08±8.14比170.54±8.25, NF-κB mRNA :0.151±0.005比0.274±0.006,NF-κB 蛋白:0.109±0.005比0.309±0.006,均 P<0.01),IL-10水平明显升高(361.94±11.04比321.80±13.86,P<0.01).光镜下可见24 h 时血必净干预组肺损伤程度明显减轻.结论血必净注射液干预能显著降低促炎因子 TNF-α、IL-6水平及 NF-κB mRNA 和蛋白的表达,提高抗炎因子 IL-10水平,减轻急性肺损伤.
目的探討血必淨註射液對硫化氫(H2S)急性中毒大鼠肺組織腫瘤壞死因子-α(TNF-α)、白細胞介素(IL-6、IL-10)及覈轉錄因子-κB(NF-κB)的影響.方法將96隻清潔級 SD 大鼠按隨機數字錶法分為正常對照組(n =8)、血必淨對照組(n =8)、H2S 染毒模型組(n =40)和血必淨榦預組(n =40).採用酶聯免疫吸附試驗(ELISA)測定肺組織中 TNF-α、IL-6、IL-10水平;用逆轉錄-聚閤酶鏈反應(RT-PCR)和蛋白質免疫印跡法(Western blotting)檢測肺組織 NF-κB mRNA 和蛋白錶達;光鏡下觀察肺組織病理學改變.結果正常對照組和血必淨對照組 TNF-α、IL-6、IL-10水平及 NF-κB mRNA 和蛋白錶達比較差異均無統計學意義(均P>0.05).與正常對照組比較,H2S 染毒模型組在染毒後2、6、12、24、48 h TNF-α(ng/L)、IL-6(ng/L)、IL-10(ng/L)水平及 NF-κB mRNA 和蛋白錶達明顯升高(均P<0.01),分彆于12、48、48、24、24 h 達峰值(TNF-α12 h:400.79±13.74,IL-648 h :242.69±8.96,IL-1048 h :353.73±10.75,NF-κB mRNA 24 h :0.568±0.007,NF-κB蛋白24 h :0.520±0.007);與 H2S 染毒模型組同期比較,血必淨榦預組 TNF-α、IL-6水平及 NF-κB mRNA和蛋白于2 h 開始即明顯降低(TNF-α:322.46±10.16比377.89±10.87,IL-6:124.08±8.14比170.54±8.25, NF-κB mRNA :0.151±0.005比0.274±0.006,NF-κB 蛋白:0.109±0.005比0.309±0.006,均 P<0.01),IL-10水平明顯升高(361.94±11.04比321.80±13.86,P<0.01).光鏡下可見24 h 時血必淨榦預組肺損傷程度明顯減輕.結論血必淨註射液榦預能顯著降低促炎因子 TNF-α、IL-6水平及 NF-κB mRNA 和蛋白的錶達,提高抗炎因子 IL-10水平,減輕急性肺損傷.
목적탐토혈필정주사액대류화경(H2S)급성중독대서폐조직종류배사인자-α(TNF-α)、백세포개소(IL-6、IL-10)급핵전록인자-κB(NF-κB)적영향.방법장96지청길급 SD 대서안수궤수자표법분위정상대조조(n =8)、혈필정대조조(n =8)、H2S 염독모형조(n =40)화혈필정간예조(n =40).채용매련면역흡부시험(ELISA)측정폐조직중 TNF-α、IL-6、IL-10수평;용역전록-취합매련반응(RT-PCR)화단백질면역인적법(Western blotting)검측폐조직 NF-κB mRNA 화단백표체;광경하관찰폐조직병이학개변.결과정상대조조화혈필정대조조 TNF-α、IL-6、IL-10수평급 NF-κB mRNA 화단백표체비교차이균무통계학의의(균P>0.05).여정상대조조비교,H2S 염독모형조재염독후2、6、12、24、48 h TNF-α(ng/L)、IL-6(ng/L)、IL-10(ng/L)수평급 NF-κB mRNA 화단백표체명현승고(균P<0.01),분별우12、48、48、24、24 h 체봉치(TNF-α12 h:400.79±13.74,IL-648 h :242.69±8.96,IL-1048 h :353.73±10.75,NF-κB mRNA 24 h :0.568±0.007,NF-κB단백24 h :0.520±0.007);여 H2S 염독모형조동기비교,혈필정간예조 TNF-α、IL-6수평급 NF-κB mRNA화단백우2 h 개시즉명현강저(TNF-α:322.46±10.16비377.89±10.87,IL-6:124.08±8.14비170.54±8.25, NF-κB mRNA :0.151±0.005비0.274±0.006,NF-κB 단백:0.109±0.005비0.309±0.006,균 P<0.01),IL-10수평명현승고(361.94±11.04비321.80±13.86,P<0.01).광경하가견24 h 시혈필정간예조폐손상정도명현감경.결론혈필정주사액간예능현저강저촉염인자 TNF-α、IL-6수평급 NF-κB mRNA 화단백적표체,제고항염인자 IL-10수평,감경급성폐손상.
Objective To investigate the effect of Xuebijing injection on the levels of tumor necrosis factor-α(TNF-α),interleukin(IL-6,IL-10)and nuclear factor-κB(NF-κB)in the lung tissue of acute hydrogen sulfide (H2S)-intoxicated rats. Methods A total of 96 Sprague-Dawley(SD)rats of clean grade were divided randomly into four groups :normal control group(n =8),Xuebijing control group(n =8),H2S-intoxicated model group(n = 40) and Xuebijing treatment group(n = 40). After modeling,the levels of TNF-α,IL-6 and IL-10 in the lung tissue were measured by enzyme-linked immunosorbent assay(ELISA)methods,and the expression of NF-κB mRNA and protein in the lung tissue was detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting methods. Pathological changes of lung tissue were observed by light microscope. Results There was no significant difference between normal control group and Xuebijing control group in the levels of TNF-α,IL-6 and IL-10 and the expression of NF-κB mRNA and protein(all P>0.05). The TNF-α(ng/L),IL-6(ng/L)and IL-10(ng/L) levels,and NF-κB mRNA and protein expression at 2,6,12,24,48 hours in rats of H2S-intoxicated model group after modeling were markedly increased than those in normal control group(all P<0.01);they reached the peak at 12, 48,48,24,24 hours respectively(TNF-α 12 hours :400.79±13.74,IL-6 48 hours :242.69±8.96,IL-10 48 hours :353.73±10.75,NF-κB mRNA 24 hours:0.568±0.007,NF-κB protein 24 hours:0.520±0.007). In comparison with the same period of H2S-intoxicated model group,the TNF-α,IL-6 levels and NF-κB mRNA and protein expression at 2 hours in rats of Xuebijing treatment group decreased significantly(TNF-α:322.46±10.16 vs. 377.89±10.87, IL-6:124.08±8.14 vs. 170.54±8.25,NF-κB mRNA:0.151±0.005 vs. 0.274±0.006,NF-κB protein:0.109±0.005 vs. 0.309±0.006,all P<0.01),and the IL-10 levels at 2 hours increased significantly(361.94±11.04 vs. 321.80±13.86,P<0.01)in the Xuebijing treatment group. And at 24 hours after modeling,the degree of lung damage was also decreased in rats of Xuebijing treatment group under the light microscope. Conclusion The interference of Xuebijing injection can significantly inhibit the levels of cytokines,TNF-α and IL-6,the expression of NF-κB mRNA and protein,and up-regulate the level of IL-10,the anti-inflammatory factor,resulting in reduction of the degree of acute lung injury in rats.
