CAJ | 학술논문

目的探讨青蒿素对脑缺血/再灌注(I/R)损伤大鼠肿瘤坏死因子-α(TNF-α)的影响.方法将40只Wistar大鼠按随机数字表法分为假手术组、模型组及青蒿素低、中、高浓度组,每组8只.采用大脑中动脉闭塞法(MCAO)复制局灶性脑I/R损伤大鼠模型.制模成功后,假手术组和模型组均灌胃30 ml生理盐水,青蒿素低、中、高浓度组分别由腹腔注射青蒿素200、300、400 mg/kg,均每日1次,连续60 d.采用放射免疫法(RIA)检测血清TNF-α含量,逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blotting)检测关节软骨组织TNF-αmRNA和蛋白表达.结果与假手术组比较,模型组和青蒿素各浓度组血清TNF-α含量和组织TNF-αmRNA及蛋白表达均明显升高;与模型组比较,青蒿素各浓度组血清TNF-α含量及组织TNF-αmRNA和蛋白表达均明显下降(均P<0.05),以青蒿素高浓度组下降更显著〔血清TNF-α(μg/L):51.9±9.4比88.3±9.7,TNF-αmRNA:0.20±0.14比0.77±0.31,TNF-α蛋白:0.19±0.08比0.76±0.09,均P<0.05〕.结论青蒿素可降低脑I/R损伤大鼠TNF-α的表达,有效控制炎症反应进程.
목적탐토청호소대뇌결혈/재관주(I/R)손상대서종류배사인자-α(TNF-α)적영향.방법장40지Wistar대서안수궤수자표법분위가수술조、모형조급청호소저、중、고농도조,매조8지.채용대뇌중동맥폐새법(MCAO)복제국조성뇌I/R손상대서모형.제모성공후,가수술조화모형조균관위30 ml생리염수,청호소저、중、고농도조분별유복강주사청호소200、300、400 mg/kg,균매일1차,련속60 d.채용방사면역법(RIA)검측혈청TNF-α함량,역전록-취합매련반응(RT-PCR)화단백질면역인적법(Western blotting)검측관절연골조직TNF-αmRNA화단백표체.결과여가수술조비교,모형조화청호소각농도조혈청TNF-α함량화조직TNF-αmRNA급단백표체균명현승고;여모형조비교,청호소각농도조혈청TNF-α함량급조직TNF-αmRNA화단백표체균명현하강(균P<0.05),이청호소고농도조하강경현저〔혈청TNF-α(μg/L):51.9±9.4비88.3±9.7,TNF-αmRNA:0.20±0.14비0.77±0.31,TNF-α단백:0.19±0.08비0.76±0.09,균P<0.05〕.결론청호소가강저뇌I/R손상대서TNF-α적표체,유효공제염증반응진정.
Objective To detect the effect of Artemisia annua on tumor necrosis factor-α(TNF-α)in rats with acute cerebral ischemia/reperfusion(I/R)injury. Methods Forty Wistar rats were randomized into sham operation group,model group,Artemisia annua high-,middle-and low-dose groups,each n=8. The model of focal cerebral I/R injury was reproduced by middle cerebral artery occlusion(MCAO). In the sham operation group and model group,30 ml normal saline was administered intra-gastrically,while in Artemisia annua low-,middle-and high-dose groups,200,300 and 400 mg/kg Artemisia annua were intra-peritoneally injected,respectively,once a day for consecutive 60 days in all the groups. The serum content of TNF-αwas detected by radioimmunoassay,and reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting methods were used to determine the expressions of tissue TNF-α mRNA and protein. Results Compared with the sham operation group,the concentration of TNF-α in serum,and the expressions of tissue TNF-α mRNA and protein in model group and various Artemisia annua groups were significantly increased;compared with the model group,the concentration of TNF-αin serum,and the expressions of tissue TNF-αmRNA and protein in various Artemisia annua groups were significantly decreased(all P<0.05),the greatest descent being in the high-dose group〔TNF-αin serum(μg/L):51.9±9.4 vs. 88.3±9.7,TNF-αmRNA:0.20±0.14 vs. 0.77±0.31,TNF-αprotein:0.19±0.08 vs. 0.76±0.09, all P<0.05〕. Conclusion Artemisia annua can down-regulate the expression of TNF-αand effectively inhibit the process of inflammation in rates with acute cerebral I/R injury.