CAJ | 학술논문

目的:研究核转录因子NF-κB在TNF-α诱导的人脐静脉血管内皮细胞中的表达,探讨NF-κB在DVT炎症-血栓反应环节的作用及中药消栓通脉颗粒的干预作用.方法:以TNF-α诱导人脐静脉内皮细胞为实验模型,分为模型组、消栓通脉高剂量组、中剂量组及低剂量组,运用RT-PCR、Western-Blot方法检测NF-κB表达量的变化.结果:人脐静脉内皮细胞经TNF-α刺激后,NF-κB的表达水平比未激活的细胞中NF-κB水平明显升高.消栓通脉颗粒含药血清干预后,NF-κB表达水平下降,且该抑制作用呈剂量依赖效应,高剂量含药血清作用下,NF-κB 表达水平最低.结论:消栓通脉颗粒能够抑制激活的人脐静脉血管细胞中NF-κB的表达.
목적:연구핵전록인자NF-κB재TNF-α유도적인제정맥혈관내피세포중적표체,탐토NF-κB재DVT염증-혈전반응배절적작용급중약소전통맥과립적간예작용.방법:이TNF-α유도인제정맥내피세포위실험모형,분위모형조、소전통맥고제량조、중제량조급저제량조,운용RT-PCR、Western-Blot방법검측NF-κB표체량적변화.결과:인제정맥내피세포경TNF-α자격후,NF-κB적표체수평비미격활적세포중NF-κB수평명현승고.소전통맥과립함약혈청간예후,NF-κB표체수평하강,차해억제작용정제량의뢰효응,고제량함약혈청작용하,NF-κB 표체수평최저.결론:소전통맥과립능구억제격활적인제정맥혈관세포중NF-κB적표체.
objective To research the expression of nuclear transcription factors NF-κB in the TNF-α in?duction of human umbilical vein endothelial cells and to evaluate the NF-κB in DVT inflammation-blood clots in the link of reaction function and Xiao Shuan Tong Mai(消栓通脉)intervention role. Methods With TNF-α induction human umbilical vein endothelial cells as experimental models, thirty rats were divided into the model group, Xiao Shuan Tong Mai(消栓通脉)high dose group, middle dose group and low dose group, The quantity changes of the NF-κB expression. RT-PCR and Western-Blot methods were used to detect. Results In human umbilical vein endothelial cells after TNF-αstimulation, the NF-κB expression level increased signifi?cantly higher than the cells unactivated NF-κB level. Under the role of serum containing Xiao Shuan Tong Mai (消栓通脉),NF-κB expression level dropped, and this inhibition was dose dependent effect. Under the role of high serum dose medication, the NF-κB expression level was minimal. Conclusion Xiao Shuan Tong Mai(消栓通脉)can inhibit the expression of NF-κB in activated human umbilical vein endothelial cells.