中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2013年
1期
43-45
,共3页
肝内胆管上皮细胞%原纤毛%淤胆%扫描电镜
肝內膽管上皮細胞%原纖毛%淤膽%掃描電鏡
간내담관상피세포%원섬모%어담%소묘전경
Cholangiocyte%cilia%cholestasis%scanning electron microscopy
目的:探讨在大鼠肝外淤胆模型中大胆管和小胆管原纤毛形态的变化.方法:将15只Wistar大鼠随机平分为未处理组(ratsctr)、胆管结扎7 d组(ratsL7)和胆管结扎10 d组(ratsL10).用胆总管结扎法制备大鼠肝外淤胆模型.利用扫描电镜技术,比较肝内大、小胆管上皮细胞原纤毛在长度和数量上的异同.结果:ratsctr组大胆管上皮细胞原纤毛平均长度为(7.76±1.14)μm,数量为每视野(10000×)(12±3)根.胆汁淤积后,大胆管上皮细胞原纤毛长度变短:ratsL7为(2.92±1.39)μm, P<0.05;ratsL10缩短为(2.34±0.37)μm,P<0.05,数量增多:ratsL7为(87±12)根, P<0.05; ratsL10为(197±46)根, P<0.05.正常大鼠小胆管上皮细胞原纤毛的平均长度为(5.15±0.89)μm,数量为每视野(4±1)根.胆汁淤积后,小胆管上皮细胞原纤毛长度变短:ratsL7为(2.52±0.96)μm, P<0.05;ratsL10为(2.31±0.53)μm, P<0.05,数量增多:ratsL7为(16±2)根, P<0.05;ratsL10为(15±3)根, P<0.05.结论:结扎胆总管导致肝脏淤胆时,胆管上皮细胞原纤毛数量增多,长度变短.
目的:探討在大鼠肝外淤膽模型中大膽管和小膽管原纖毛形態的變化.方法:將15隻Wistar大鼠隨機平分為未處理組(ratsctr)、膽管結扎7 d組(ratsL7)和膽管結扎10 d組(ratsL10).用膽總管結扎法製備大鼠肝外淤膽模型.利用掃描電鏡技術,比較肝內大、小膽管上皮細胞原纖毛在長度和數量上的異同.結果:ratsctr組大膽管上皮細胞原纖毛平均長度為(7.76±1.14)μm,數量為每視野(10000×)(12±3)根.膽汁淤積後,大膽管上皮細胞原纖毛長度變短:ratsL7為(2.92±1.39)μm, P<0.05;ratsL10縮短為(2.34±0.37)μm,P<0.05,數量增多:ratsL7為(87±12)根, P<0.05; ratsL10為(197±46)根, P<0.05.正常大鼠小膽管上皮細胞原纖毛的平均長度為(5.15±0.89)μm,數量為每視野(4±1)根.膽汁淤積後,小膽管上皮細胞原纖毛長度變短:ratsL7為(2.52±0.96)μm, P<0.05;ratsL10為(2.31±0.53)μm, P<0.05,數量增多:ratsL7為(16±2)根, P<0.05;ratsL10為(15±3)根, P<0.05.結論:結扎膽總管導緻肝髒淤膽時,膽管上皮細胞原纖毛數量增多,長度變短.
목적:탐토재대서간외어담모형중대담관화소담관원섬모형태적변화.방법:장15지Wistar대서수궤평분위미처리조(ratsctr)、담관결찰7 d조(ratsL7)화담관결찰10 d조(ratsL10).용담총관결찰법제비대서간외어담모형.이용소묘전경기술,비교간내대、소담관상피세포원섬모재장도화수량상적이동.결과:ratsctr조대담관상피세포원섬모평균장도위(7.76±1.14)μm,수량위매시야(10000×)(12±3)근.담즙어적후,대담관상피세포원섬모장도변단:ratsL7위(2.92±1.39)μm, P<0.05;ratsL10축단위(2.34±0.37)μm,P<0.05,수량증다:ratsL7위(87±12)근, P<0.05; ratsL10위(197±46)근, P<0.05.정상대서소담관상피세포원섬모적평균장도위(5.15±0.89)μm,수량위매시야(4±1)근.담즙어적후,소담관상피세포원섬모장도변단:ratsL7위(2.52±0.96)μm, P<0.05;ratsL10위(2.31±0.53)μm, P<0.05,수량증다:ratsL7위(16±2)근, P<0.05;ratsL10위(15±3)근, P<0.05.결론:결찰담총관도치간장어담시,담관상피세포원섬모수량증다,장도변단.
Objective To study the morphology of cholangiocyte cilia in large bile ducts (LBDs) and small bile ducts (SBDs) of rats with extra-hepatic cholestasis induced by bile duct ligation (BDL). Methods Fifteen rats were divided randomly into three groups (5 in each). Rats in these groups were subjected to 7-day BDL (rat?sL7), 10-day BDL (ratsL10) and no special treatment (ratsctr). We characterized the primary cilia of cholangiocytes in these rats using scanning electron microscope. Results In the large bile ducts of ratsctr, the average length and number of primary cilia were(7.76 ± 1.14)μm and(12 ± 3)/field, respectively. Compared to the control val?ues, the primary cilia in LBDs were decreased in length (ratsL7 2.92 ± 1.39, P<0.05 and ratsL10 2.34 ± 0.37, P<0.05) and increased in numbers (ratsL7 87 ± 12/field, P<0.05 and ratsL10 197 ± 46/field, P<0.05) as a result of BDL. In the SBDs of ratsctr, the average length and number of primary cilia were 5.15 ± 0.89 μm and 4 ± 1/field, respectively. Compared to the control values, the primary cilia in SBDs were decreased in length (ratsL7 2.52 ± 0.96, P<0.05 and ratsL10 2.31 ± 0.53, P<0.05) and increased in numbers (ratsL7 16 ± 2/field, P<0.05 and ratsL10 15 ± 3/field, P<0.05) as a result of BDL. Conclusion After rat BDL, cholangiocyte cil?ia were increased in number and decreased in length.
