CAJ | 학술논문

目的通过动物实验验证组蛋白去乙酰化酶抑制剂丁酸钠是否可以保护耳蜗毛细胞免受庆大霉素引起的耳毒性损害.方法:10只听力正常豚鼠被用于本实验.向每只动物右耳的圆窗龛放置小块明胶海绵(0.3 mm3)并注入10μl丁酸钠(100 mg/ml)溶液,左耳圆窗龛内置放的明胶海绵则注入10μl人工外淋巴液作为对照.手术后第三天开始按照每公斤体重200mg的剂量每天一次性对豚鼠肌肉注射庆大霉素,连续用药5天.在停药后第3天检测听性脑干诱发电位,常规制备全耳蜗基底膜铺片结合鬼笔环肽染色,对全耳蜗毛细胞进行计数并制备全耳蜗毛细胞图.结果:圆窗龛置放丁酸钠溶液组的听性脑干诱发电位阈值比圆窗龛置放外淋巴液组低,两组资料相比有显著性统计学差异(P<0.01),圆窗龛置放丁酸钠溶液组的耳蜗毛细胞缺失程度比圆窗龛置放外淋巴液组轻.结论:组蛋白去乙酰化酶抑制剂丁酸钠对庆大霉素导致的豚鼠耳聋和耳蜗损害具有一定的保护作用.
목적통과동물실험험증조단백거을선화매억제제정산납시부가이보호이와모세포면수경대매소인기적이독성손해.방법:10지은력정상돈서피용우본실험.향매지동물우이적원창감방치소괴명효해면(0.3 mm3)병주입10μl정산납(100 mg/ml)용액,좌이원창감내치방적명효해면칙주입10μl인공외림파액작위대조.수술후제삼천개시안조매공근체중200mg적제량매천일차성대돈서기육주사경대매소,련속용약5천.재정약후제3천검측은성뇌간유발전위,상규제비전이와기저막포편결합귀필배태염색,대전이와모세포진행계수병제비전이와모세포도.결과:원창감치방정산납용액조적은성뇌간유발전위역치비원창감치방외림파액조저,량조자료상비유현저성통계학차이(P<0.01),원창감치방정산납용액조적이와모세포결실정도비원창감치방외림파액조경.결론:조단백거을선화매억제제정산납대경대매소도치적돈서이롱화이와손해구유일정적보호작용.
Objective To investigate the protective effects of histone deacetylase (HDAC) inhibitor sodium butyrate (NaB) against gentamicin (GM) ototoxicity in vivo. Methods Ten guinea pigs with normal hearing response were used for this study. A small piece of gelatin sponge (0.3 mm3) was surgically placed in the round window niche in both ears. NaB (100 mg/ml, 10μl) was infused into the gelatin in the right round window, whereas artificial perilymph (10μl)was infused into the gelatin in the left round window niche as control. Three days after surgery, animals received intramuscular injection of gentamicin (200mg/kg/day) for 5 consecutive days. The threshold of Auditory Brainstem Responses (ABRs) was measured 3 days after the final GM injection. Cochlear surface preparations were made with palloidin staining to determine the num?ber of missing cochlear hair cells as a pattern of cochleogram. Results Compared to the left ear that was treated with artifi?cial perilymph, lower thresholds of ABR and less hair cell loss were seen in NaB treated ears. Conclusion NaB may allevi?ate gentamicin-induced cochlear lesions.