CAJ | 학술논문

目的探讨树突状细胞(DCs)是否及通过何种途径参与内耳的免疫应答,分析中耳炎免疫引发的内耳免疫对内耳功能的影响.方法将Hoechst33342标记的DCs分别经由脑脊液,颈外静脉及颈部皮下注入豚鼠体内,并在无菌条件下于右耳经鼓膜注射1×108?L-1的金葡菌液100μL,左耳作正常对照,造急性中耳炎豚鼠模型.3天后基底膜切片及铺片若单明-鬼笔环肽(Phalloidin)染色,荧光显微镜(Olympus)及共聚焦显微镜观察.结果三种途径注入的DCs均可以在中耳炎豚鼠的中耳粘膜发现.在豚鼠内耳前庭阶、鼓阶内可以发现大量的免疫细胞渗入,同时可见DC位于内耳,数目很少,分布于基底膜、血管纹、螺旋神经节及壶腹嵴.三组动物对照耳仅皮下注射组有一例在耳蜗发现DCs渗入.器官切片除脑脊液组在脑中发现DC外,其余均未见DCs渗入.结论DCs可以通过不同途径参与中耳炎所致的内耳免疫反应.作为最强的抗原提呈细胞,免疫反应的启动者,DCs在内耳诱导强烈的免疫反应,这种免疫反应可能对内耳的结构和功能产生不可逆的严重损伤,DCs功能紊乱可能为自身免疫性内耳疾病的原因之一.提示抑制过度免疫反应能够有效保护内耳的免疫损伤.
목적탐토수돌상세포(DCs)시부급통과하충도경삼여내이적면역응답,분석중이염면역인발적내이면역대내이공능적영향.방법장Hoechst33342표기적DCs분별경유뇌척액,경외정맥급경부피하주입돈서체내,병재무균조건하우우이경고막주사1×108?L-1적금포균액100μL,좌이작정상대조,조급성중이염돈서모형.3천후기저막절편급포편약단명-귀필배태(Phalloidin)염색,형광현미경(Olympus)급공취초현미경관찰.결과삼충도경주입적DCs균가이재중이염돈서적중이점막발현.재돈서내이전정계、고계내가이발현대량적면역세포삼입,동시가견DC위우내이,수목흔소,분포우기저막、혈관문、라선신경절급호복척.삼조동물대조이부피하주사조유일례재이와발현DCs삼입.기관절편제뇌척액조재뇌중발현DC외,기여균미견DCs삼입.결론DCs가이통과불동도경삼여중이염소치적내이면역반응.작위최강적항원제정세포,면역반응적계동자,DCs재내이유도강렬적면역반응,저충면역반응가능대내이적결구화공능산생불가역적엄중손상,DCs공능문란가능위자신면역성내이질병적원인지일.제시억제과도면역반응능구유효보호내이적면역손상.
Objective To study the influence on morphology and function by DCs injected into the inner ear via different routes in guinea pigs with otitis media.Methods: DCs labeled with Hoechst33342 were injected through the cerebrospinal fluid, the external jugular vein and neck skin respectively in guinea pigs. The middle ear cavity on right side was inoculated with 0.1ml 1×108/ml staphloccoccosis aureus, with the left ear serving as the control. Changes in the cochlea were examined 3 days after infection by fluorescence microscopy and confocal microscopy. Results DCs injected via all three routes were found in the middle ear mucosa in these guinea pigs with otitis media. DCs also were seen in the base?ment membrane, spiral ligament, stria vascularis, spiral ganglion and crista ampullaris, but the number was extremely low. In only one case DCs infiltration was seen in the cochlea on the control side. DCs infiltration in the brain was seen only in the cerebrospinal fluid injection group. Conclusion Dendritic cells are involved in the inner ear immune response to oti?tis media through all administration routes tested in this study. DCs are unique APCs and have been referred to as“pro?fessional”APCs. DCs have the ability to induce a primary immune response in the inner ear. Immune reaction can irre?versibly damage the structure and function of the inner ear. Our study suggests that early use of steroids may be effective in protecting the inner ear from immune damage.