CAJ | 학술논문

采用高效液相色谱法测定绿花椰菜水提物中莱菔硫烷的含量;以C57BL/6小鼠建立急性酒精性肝损伤模型,用苏木精‐伊红染色法( hematoxylin‐eosin staining , HE )检测莱菔硫烷对小鼠肝脏病理学的影响;采用半自动生化分析仪测定莱菔硫烷对肝脏丙氨酸氨基转移酶( alanine aminotransferase , ALT )、天门冬氨酸氨基转移酶( aspartate aminotransferase , AST)和碱性磷酸酶( alkaline phosphatase , ALP)活性的影响,以探讨绿花椰菜中的活性成分莱菔硫烷对急性酒精性肝损伤的保护作用畅结果表明:绿花椰菜水提物中莱菔硫烷的含量为1.26 mg/片;莱菔硫烷各剂量组均可明显降低酒精所致的肝质量增加,肝/体比值较酒精模型组明显下降( P<0.01);莱菔硫烷各剂量组均能减轻酒精对肝脏的病理学损伤,其中,高剂量莱菔硫烷组(80 mg/kg)的作用尤为明显;莱菔硫烷能明显抑制酒精对肝脏ALT、AST和ALP等特征性酶的诱导作用,其中40和80 mg/kg中、高剂量莱菔硫烷的保护作用更明显畅说明莱菔硫烷能保护酒精对肝脏的损伤.
채용고효액상색보법측정록화야채수제물중래복류완적함량;이C57BL/6소서건립급성주정성간손상모형,용소목정‐이홍염색법( hematoxylin‐eosin staining , HE )검측래복류완대소서간장병이학적영향;채용반자동생화분석의측정래복류완대간장병안산안기전이매( alanine aminotransferase , ALT )、천문동안산안기전이매( aspartate aminotransferase , AST)화감성린산매( alkaline phosphatase , ALP)활성적영향,이탐토록화야채중적활성성분래복류완대급성주정성간손상적보호작용창결과표명:록화야채수제물중래복류완적함량위1.26 mg/편;래복류완각제량조균가명현강저주정소치적간질량증가,간/체비치교주정모형조명현하강( P<0.01);래복류완각제량조균능감경주정대간장적병이학손상,기중,고제량래복류완조(80 mg/kg)적작용우위명현;래복류완능명현억제주정대간장ALT、AST화ALP등특정성매적유도작용,기중40화80 mg/kg중、고제량래복류완적보호작용경명현창설명래복류완능보호주정대간장적손상.
@@@@Summary Sulforaphane ( SFN) is an isothiocyanate ( ITC) which exists as a precursor of glucosinolate ( GS) in various cruciferous vegetables especially in broccoli . Sulforaphane has been regarded as a potential of anti‐cancer agent derived from diet , mostly because of its powerful induction of phase Ⅱ enzymes , and it is also acted as an antagonist of injury factors including lipopolysaccharide ( LPS) and homocysteine ( HCY) . The present study aims to explore whether broccoli extract , rich in sulforaphane , can protect the acute liver injury induced by ethanol in mice or not . The quantity of sulforaphane in broccoli extract was detected by high performance liquid chromatography ( HPLC) . Hematoxylin‐eosin ( HE) staining was used to determine the pathological changes in C 57BL/6 mice model with the acute liver injury induced by ethanol . The activities of alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) and alkaline phosphatase ( ALP) in serum of mice were measured using semi‐automatic biochemical analyzer . The results showed that the quantity of sulforaphane in the broccoli extract was 1 .26 mg/tablet . Liver masses in all sulforaphane treatment groups were obviously reduced which were presented as the sharply decreased ratio of liver mass/body mass . The seriously pathological evidence was observed in ethanol treatment group , while less changes were seen in the sulforaphane treatment groups . Sulforaphane could dramatically inhibit the activities of ALT , AST and ALP in serum of mice induced by ethanol , with more obvious suppression in the moderate ( 40 mg/kg) and high‐dose (80 mg/kg) groups , respectively . The result above indicates that the sulforaphane from broccoli extract tablets is able to protect the liver injury induced by ethanol .