神经药理学报
神經藥理學報
신경약이학보
Journal of Hebei North University(Medical Edition)
2011年
4期
12-15
,共4页
张美蓉%魏守蓉%吴燕川%孙芳玲%艾厚喜%张丽%王文
張美蓉%魏守蓉%吳燕川%孫芳玲%艾厚喜%張麗%王文
장미용%위수용%오연천%손방령%애후희%장려%왕문
连翘苷%1-甲基-4-苯基吡啶离子%SH-SY5Y细胞%神经保护
連翹苷%1-甲基-4-苯基吡啶離子%SH-SY5Y細胞%神經保護
련교감%1-갑기-4-분기필정리자%SH-SY5Y세포%신경보호
Phillyrin%1-methyl-4-phenylpyridinium%SH-SY5Y cells%neuroprotective
目的:研究连翘苷对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinium,MPP+)诱导人神经母细胞株SH-SY5Y细胞损伤的神经保护作用.方法:培养的SH-SY5Y细胞加入不同浓度的连翘苷,以MTT代谢率为指标确定连翘苷的无毒范围;对培养的SH-SY5Y细胞加入连翘苷100,10,1μmol·L-1预孵育24 h,加入1 mmol·L-1 MPP+作用48 h诱导损伤,观察MTT代谢率、乳酸脱氢酶(Lactate dehydrogenase,LDH)漏出率的改变.结果:连翘苷在1~100μmol·L-1浓度范围内不影响SH-SY5Y细胞存活率;与正常对照组细胞相比, MPP+损伤模型组MTT代谢率明显降低(P<0.001),LDH漏出率显著增加(P<0.001);与模型组相比,连翘苷各组细胞活性显著升高(P<0.05),LDH漏出率明显降低(P<0.01).结论:连翘苷对MPP+诱导的SH-SY5Y细胞损伤具有神经保护作用.
目的:研究連翹苷對1-甲基-4-苯基吡啶離子(1-methyl-4-phenylpyridinium,MPP+)誘導人神經母細胞株SH-SY5Y細胞損傷的神經保護作用.方法:培養的SH-SY5Y細胞加入不同濃度的連翹苷,以MTT代謝率為指標確定連翹苷的無毒範圍;對培養的SH-SY5Y細胞加入連翹苷100,10,1μmol·L-1預孵育24 h,加入1 mmol·L-1 MPP+作用48 h誘導損傷,觀察MTT代謝率、乳痠脫氫酶(Lactate dehydrogenase,LDH)漏齣率的改變.結果:連翹苷在1~100μmol·L-1濃度範圍內不影響SH-SY5Y細胞存活率;與正常對照組細胞相比, MPP+損傷模型組MTT代謝率明顯降低(P<0.001),LDH漏齣率顯著增加(P<0.001);與模型組相比,連翹苷各組細胞活性顯著升高(P<0.05),LDH漏齣率明顯降低(P<0.01).結論:連翹苷對MPP+誘導的SH-SY5Y細胞損傷具有神經保護作用.
목적:연구련교감대1-갑기-4-분기필정리자(1-methyl-4-phenylpyridinium,MPP+)유도인신경모세포주SH-SY5Y세포손상적신경보호작용.방법:배양적SH-SY5Y세포가입불동농도적련교감,이MTT대사솔위지표학정련교감적무독범위;대배양적SH-SY5Y세포가입련교감100,10,1μmol·L-1예부육24 h,가입1 mmol·L-1 MPP+작용48 h유도손상,관찰MTT대사솔、유산탈경매(Lactate dehydrogenase,LDH)루출솔적개변.결과:련교감재1~100μmol·L-1농도범위내불영향SH-SY5Y세포존활솔;여정상대조조세포상비, MPP+손상모형조MTT대사솔명현강저(P<0.001),LDH루출솔현저증가(P<0.001);여모형조상비,련교감각조세포활성현저승고(P<0.05),LDH루출솔명현강저(P<0.01).결론:련교감대MPP+유도적SH-SY5Y세포손상구유신경보호작용.
Objective:To investigate the effects of Phillyrin on 1-methyl-4-phenylpyridini-um (MPP+)-induced injury in SH-SY5Y neuroblastoma cells. Methods:SH-SY5Y cells were in-cubated with Phillyrin of different concentrations. MTT metabolic rate was measured as the cell survival rate to determine the effective concentrations of Phillyrin. After SH-SY5Y cells were incubated with Phillyrin (1,10,100μmol·L-1) for 24 h prior to exposure to MPP+(1 mmol·L-1) for 48 h,the MTT metabolic rate and LDH leakage rate were observed. Results:Phillyrin did not decrease the survival rate of SH-SY5Y cells at the concentrations from 1 to 100μmol·L-1. Com-pared with the control group,MPP+ decreased the MTT metabolic rate(P<0.001)and increased the LDH leakage rate (P<0.001) of SH-SY5Y cells. However,Phillyrin significantly increased the cell activity (P<0.05) and decreased LDH leakage rate of SH-SY5Y cells with MPP+-induced injury. Conclusion:Phillyrin protects SH-SY5Y cells against MPP+-induced cytotoxicity.