CAJ | 학술논문

目的分析比较微柱凝胶法与传统抗人球蛋白法检测孕妇 IgG 抗 A(B)抗体效价的相关性和线性数量关系,推算相应的微柱凝胶法参考值.方法分别采用微柱凝胶法和传统抗人球蛋白法检测199例门诊和住院 O 型孕妇 IgG 抗 A(B)效价,并结合其中58例新生儿 ABO 溶血病检测结果进行统计分析.结果微柱凝胶法检测阳性率达79.9%,传统抗人球蛋白法阳性率达64.8%,两者有统计学差异(P<0.01),两种方法的结果呈高度正相关(r=0.869,P<0.01).推算微柱凝胶法相应的参考理论效价为114,与新生儿溶血病临床资料分析较相符.结论本研究推算的理论参考效价有助于今后微柱凝胶法医学决定值的建立.
목적분석비교미주응효법여전통항인구단백법검측잉부 IgG 항 A(B)항체효개적상관성화선성수량관계,추산상응적미주응효법삼고치.방법분별채용미주응효법화전통항인구단백법검측199례문진화주원 O 형잉부 IgG 항 A(B)효개,병결합기중58례신생인 ABO 용혈병검측결과진행통계분석.결과미주응효법검측양성솔체79.9%,전통항인구단백법양성솔체64.8%,량자유통계학차이(P<0.01),량충방법적결과정고도정상관(r=0.869,P<0.01).추산미주응효법상응적삼고이론효개위114,여신생인용혈병림상자료분석교상부.결론본연구추산적이론삼고효개유조우금후미주응효법의학결정치적건립.
[ ] Objective To calculate the reference value of anti-A (B) IgG antibody in saerum of pregnant women with mi-crocolumn gel assay. Methods The methods of microcolumn gel assay and traditional Coomb's test were used to measure the titer of anti-A (B) IgG in 199 pregnant women of blood type O. Combined with the test results of 58 cases of newborn ABO hemolytic diseases the results were analyzed by statistics software SPSS 18.0. Results The positive rate of microcolumn gel as-say was 79.9%, while that of traditional Coomb's test was 64.8%(P<0.01); Pearson Chi-square test showed a highly positive cor-relation between the two methods (r=0.869,P<0.01). The calculated reference value of microcolumn gel assay was 114, also was consistent with clinical data analysis. Conclusion The calculating reference titer of anti-A (B) IgG wil be of value for application of microcolumn gel assay in future research and clinical practice.