CAJ | 학술논문

目的建立细菌脂肪酸气相色谱分析技术对创伤弧菌(vibrio vulnificus, Vv)鉴定的新方法.方法 Vv经35℃、24 h血平板培养,收集菌落,皂化、甲基化、萃取、洗涤步骤,采用气相色谱法测定 Vv细菌脂肪酸的种类与含量结果,并与软件标准图谱进行比对与识别.结果20min 内色谱给出 Vv脂肪酸特征图,峰位与标准图谱的差异仅为0.21%,99.17%的峰能明确识别,Vv 的脂肪酸种类与含量为 C16:0(29.66%),C16:1ω7C/C16:1ω6C(28.32%),C18:1ω7C(13.42%),C14:0(5.41%),C14:03OH/C16:1 iso1(4.31%)和 C12:03OH(4.01%).结论细菌脂肪酸的种类与数量可作为细菌鉴定特征,气相色谱分析技术具有灵敏、精确、快速特点,与经典的细菌生化反应及 DNA 鉴定相比,具有生物安全、成本低廉等优点,为临床实验室细菌的鉴定开辟了新途径.
목적건립세균지방산기상색보분석기술대창상호균(vibrio vulnificus, Vv)감정적신방법.방법 Vv경35℃、24 h혈평판배양,수집균락,조화、갑기화、췌취、세조보취,채용기상색보법측정 Vv세균지방산적충류여함량결과,병여연건표준도보진행비대여식별.결과20min 내색보급출 Vv지방산특정도,봉위여표준도보적차이부위0.21%,99.17%적봉능명학식별,Vv 적지방산충류여함량위 C16:0(29.66%),C16:1ω7C/C16:1ω6C(28.32%),C18:1ω7C(13.42%),C14:0(5.41%),C14:03OH/C16:1 iso1(4.31%)화 C12:03OH(4.01%).결론세균지방산적충류여수량가작위세균감정특정,기상색보분석기술구유령민、정학、쾌속특점,여경전적세균생화반응급 DNA 감정상비,구유생물안전、성본저렴등우점,위림상실험실세균적감정개벽료신도경.
[ ] Objective To establish a new method for identification of Vibrio vulnificus (Vv) by fatty acid analysis with gas chromatography (GC). Methods Vv strains were cultivated on sheep blood agar at 35℃ for 24h. By harvesting, saponification, methylation, extraction and basic wash steps. The whole cel ular fatty acids were detected by gas chromatography. The peak position of results were matched with reference chromatograms. Results The identification of Vv were accomplished within 20 minutes by rapid analysis; and 99.17% of the peaks were identified with a peak position error rate of 0.21%. Bacteria extracts contained straight and branched fatty acids with 9 to 20 carbon lengths. Strain Vv contained C16:0 (29.66% ), C16:1ω7C/C16:1ω6C (28.32%), C18:1ω7C(13.42%), C14:0(5.41%), C14:0 3OH/C16:1iso1(4.31%) and C12:0 3OH (4.01%) as the major fatty acids. Conclusion A novel fatty acid analysis method was established for identification of Vv species and sub-species, which is sensitive, accurate, rapid and safer than conventional biochemical/ DNA-based identification methods.