CAJ | 학술논문

目的探讨低蛋白配伍α酮酸饮食对糖尿病大鼠肾组织 TGF-β及其受体表达的影响.方法将实验大鼠随机分为:健康大鼠对照组[对照组,给予正常蛋白饮食(含18.5%酪蛋白)]、糖尿病模型组(NPD 组,给予正常蛋白饮食);糖尿病模型低蛋白饮食组[LPD 组,给予低蛋白饮食(含6%酪蛋白)];糖尿病模型低蛋白配伍α酮酸饮食组[LK 组,给予低蛋白配伍α酮酸饮食(含5%酪蛋白+1%α酮酸)].每组8只,饲养12周.收集血、尿标本,检测生化指标及24h 尿蛋白定量;取肾组织标本,实时定量 PCR 法检测 TGF-β1及Ⅰ型受体(TβRI)、Ⅱ型受体(TβRⅡ)mRNA 水平.结果 NPD 组 Scr、BUN 及24h 尿蛋白定量水平均明显高于LPD 组和 LK 组(均 P<0.05).LPD 组和 LK 组肾组织 TGF-β1及 TβRI、TβRⅡ mRNA 基因表达水平均较 NPD 组明显下调(均 P<0.05),且 LK 组较 LPD 组下调更显著(P<0.05).结论低蛋白配伍α酮酸饮食可能通过下调肾组织 TGF-β1及其受体 TβRI、TβRⅡ mRNA 基因表达水平保护肾功能,减少蛋白尿,延缓糖尿病肾病进展.
목적탐토저단백배오α동산음식대당뇨병대서신조직 TGF-β급기수체표체적영향.방법장실험대서수궤분위:건강대서대조조[대조조,급여정상단백음식(함18.5%락단백)]、당뇨병모형조(NPD 조,급여정상단백음식);당뇨병모형저단백음식조[LPD 조,급여저단백음식(함6%락단백)];당뇨병모형저단백배오α동산음식조[LK 조,급여저단백배오α동산음식(함5%락단백+1%α동산)].매조8지,사양12주.수집혈、뇨표본,검측생화지표급24h 뇨단백정량;취신조직표본,실시정량 PCR 법검측 TGF-β1급Ⅰ형수체(TβRI)、Ⅱ형수체(TβRⅡ)mRNA 수평.결과 NPD 조 Scr、BUN 급24h 뇨단백정량수평균명현고우LPD 조화 LK 조(균 P<0.05).LPD 조화 LK 조신조직 TGF-β1급 TβRI、TβRⅡ mRNA 기인표체수평균교 NPD 조명현하조(균 P<0.05),차 LK 조교 LPD 조하조경현저(P<0.05).결론저단백배오α동산음식가능통과하조신조직 TGF-β1급기수체 TβRI、TβRⅡ mRNA 기인표체수평보호신공능,감소단백뇨,연완당뇨병신병진전.
[ ] Objective To investigate the effects of low protein diet with α-keto acid supplemented on renal expression of TGF-β and its receptors type I (TβRI) and type Ⅱ(TβRⅡ) mRNA in diabetic rats. Methods Diabetes was induced in 24 male SD rats, then the animals were randomly assigned to three groups with 8 in each group: normal protein group (NPD, fed with 18.5% casein protein), low protein group (LPD, fed with 6% casein protein) and low protein with α-keto acid supplemented group (LK, fed with 5% casein protein+1% α-keto acid). The rats were sacrificed at the end of 12th week, serum creatinine, blood urea, serum albumin and 24-h urinary protein excretion were measured. Real-time PCR was used to detect the mRNA ex-pression of TGF-β1, TβRI and TβRII. Results Serum creatinine, blood urea and proteinuria of NPD group were significantly higher than those of LPD and LK groups(P<0.05). Expression of TGF-β1, TβRI and TβRⅡmRNAs in LPD and LK groups were significantly lower than those in NPD group(P<0.05), and those in LK group were lower than in LPD group(P<0.05). Conclu-sion Low protein diet with α-keto acid supplemented exhibits renal protective effects, which might be related to the down-regulating expression of TGF-β1 and its receptors.