CAJ | 학술논문

目的通过对阳离子聚合物聚乙烯亚胺(PEI)以及 PEI 联合壳聚糖介导 pGL3质粒细胞内转染的效率及细胞毒性的观察,为其进一步携载目的基因转染细胞做好前期铺垫工作.方法培养 HaCaT 及 HUVEC 细胞,同时制备壳聚糖-PEI600Da (CP)以及 CP/DNA、PEI/DNA 纳米粒,采用 MTT 法观察壳聚糖、PEI600Da、PEI25kDa、CP 以及 CP/DNA、PEI/DNA 纳米粒对上述细胞的毒性作用,采用虫荧光素酶检测法观察 CP/DNA、PEI/DNA 纳米粒的转染效率.结果 CP/DNA、PEI600Da/DNA 纳米粒的细胞毒性明显低于 PEI25kDa/DNA 纳米粒的细胞毒性;CP/DNA、PEI600Da/DNA 纳米粒明显提高 DNA 转染 HaCaT、HUVEC 细胞的效率.结论 CP/DNA、PEI600Da/DNA 纳米粒有效转染 HaCaT、HUVEC 细胞,且细胞毒性低;阳离子聚合物 CP、PEI600Da 作为转染基因转染的载体具有广阔的应用前景.
목적통과대양리자취합물취을희아알(PEI)이급 PEI 연합각취당개도 pGL3질립세포내전염적효솔급세포독성적관찰,위기진일보휴재목적기인전염세포주호전기포점공작.방법배양 HaCaT 급 HUVEC 세포,동시제비각취당-PEI600Da (CP)이급 CP/DNA、PEI/DNA 납미립,채용 MTT 법관찰각취당、PEI600Da、PEI25kDa、CP 이급 CP/DNA、PEI/DNA 납미립대상술세포적독성작용,채용충형광소매검측법관찰 CP/DNA、PEI/DNA 납미립적전염효솔.결과 CP/DNA、PEI600Da/DNA 납미립적세포독성명현저우 PEI25kDa/DNA 납미립적세포독성;CP/DNA、PEI600Da/DNA 납미립명현제고 DNA 전염 HaCaT、HUVEC 세포적효솔.결론 CP/DNA、PEI600Da/DNA 납미립유효전염 HaCaT、HUVEC 세포,차세포독성저;양리자취합물 CP、PEI600Da 작위전염기인전염적재체구유엄활적응용전경.
Objective To assess the efficiency and toxicity of polyethylene imine (PEI) and chiosan (CS) as non-viral vecors in PGL3 plasmid cel transfection. Methods Chiosan-PEI600Da (CP), CP/DNA and PEI/DNA nanoparticles were pre-pared. The toxicity of CS, PEI600Da, PEI25Kda, CP and CP/DNA, PEI/DNA nanoparticles on the cultured HUVEC and HaCaT cel s were tested by MTT method. The transfection effiency of CP/DNA and PEI/DNA was assessed with insect enzyme fluorescent el-ement method. Results The toxicity of CP, PEI600Da, CP/DNA, PEI600Da/DNA nanoparticles on HUVEC and HaCaT cells were much lower than that of PEI25Kda and PEI25KDa/DNA nanoparticles. CP/DNA and PEI600Da/DNA nanoparticles significantly in-creased the efficiency of DNA transfection in HUVEC and HaCaT cel s. Conclusion CP/DNA and PEI600Da/DNA nanoparticles can effectively transfect PGL3 plasmid into HUVEC and HaCaT cel s with low toxicity.