CAJ | 학술논문

目的利用高糖、高脂饮食联合注射链脲佐菌素(STZ),建立 SD 大鼠2型糖尿病动物模型,为抗糖尿病药物的筛选以及实验提供一个良好的动物模型.方法35只雄性 SD 大鼠随机分为实验组和对照组,实验组(25只)饲喂高糖、高脂饲料,对照组(10只)饲喂基础饲料.饲喂4周后,按30mg/kg 剂量一次性腹腔注射 STZ,对照组注射同等剂量的柠檬酸缓冲液.实验组继续饲喂高糖、高脂饲料,注射后每周采血1次,连续采3周,比较实验组与对照组空腹血糖、INS、血脂的变化.注射 STZ 后3周,剖杀所有实验大鼠,取胰腺,显微镜下观察胰岛的变化.结果实验组大鼠2型糖尿病特征显著,成功率高达88%.与对照组相比,实验组大鼠空腹血糖、INS、TC、TG、LDL 以及 HOMA-IR 明显升高(P<0.05或0.01).结论高糖、高脂饮食加以一次性注射小剂量 STZ 可成功建立 SD 大鼠2型糖尿病模型.
목적이용고당、고지음식연합주사련뇨좌균소(STZ),건립 SD 대서2형당뇨병동물모형,위항당뇨병약물적사선이급실험제공일개량호적동물모형.방법35지웅성 SD 대서수궤분위실험조화대조조,실험조(25지)사위고당、고지사료,대조조(10지)사위기출사료.사위4주후,안30mg/kg 제량일차성복강주사 STZ,대조조주사동등제량적저몽산완충액.실험조계속사위고당、고지사료,주사후매주채혈1차,련속채3주,비교실험조여대조조공복혈당、INS、혈지적변화.주사 STZ 후3주,부살소유실험대서,취이선,현미경하관찰이도적변화.결과실험조대서2형당뇨병특정현저,성공솔고체88%.여대조조상비,실험조대서공복혈당、INS、TC、TG、LDL 이급 HOMA-IR 명현승고(P<0.05혹0.01).결론고당、고지음식가이일차성주사소제량 STZ 가성공건립 SD 대서2형당뇨병모형.
[ ] Objective To establish type 2 diabetic model in rats. Methods Thirty five male SD rats were randomly divid-ed into model group (n=25) and control group (n=10). The rats in model group were fed with high-carbonhydrate-fat diet and rats in control group were fed with basic diet. After 4 weeks, the rats in model group received one-off intraperitoneal injection of streptozotocin at a dose of 30 mg/(kg·BW); and the control group was injected with the same volume of citrate sodium buffer. Af-ter injection the model group was fed continual y with high-carbonhydrate-fat feeds, and the blood samples were col ected for measurement of fasting blood glucose, serum insulin and blood fat in the first, second and third week. Al animals were sacrificed and pancreatic glands were col ected for histopathological examination. Results At the end of experiments 88% rats in model group presented characteristic diabetic manifestations. The blood glucose levels (P<0.01), serum insulin levels (P<0.01), total cholesterol (P<0.05), triglyceride (P<0.05), low density lipoprotein (P<0.05) and insulin resistance index (P<0.05) in the model group were increased significantly compared with the control group. Conclusion Type 2 diabetic model has been established successful y by feeding with high-carbonhydrate-fat diet and one-off intraperitoneal injection with streptozotocin in SD rats.