CAJ | 학술논문

目的探讨急性脑梗死患者血清血小板衍生内皮细胞生长因子(PD-ECGF)和血管内皮细胞生长因子(VEGF)的动态变化并分析与梗死灶大小、梗死病因类型的相关性.方法采用双抗体夹心酶联免疫吸附法(ELISA)动态测定34例急性脑梗死患者在发病后第1、3、7、14天时血清 PD-ECGF 和 VEGF 的浓度,对照组为30例本院健康体检者.并根据体积大小分为3 组:大梗死灶组10例,中等梗死灶组13例和小梗死灶组11例.根据病因学分为大动脉粥样硬化组15例,小血管病变组10例和心源性组9例.分析血清 PD-ECGF 和 VEGF 浓度变化与梗死灶大小、梗死病因类型相关性.结果急性脑梗死患者在中风后各时间点的血清PD-ECGF 和 VEGF 浓度均明显增高(均 P<0.05),其中血清 PD-ECGF 浓度在中风后3d 达高峰,血清 VEGF 浓度在中风后7d 达高峰,两组浓度在中风后14d 一直维持增高.血清 PD-ECGF 和 VEGF 浓度在大梗死灶组和大动脉粥样硬化组中增高明显,差异均有统计学意义(均 P<0.05).结论急性脑梗死患者血清 PD-ECGF 和 VEGF 浓度有明显的增高,升高程度和梗死杜大小、病因类型相关,血清 PD-ECGF 浓度达峰时间早于血清 VEGF 浓度达峰时间.PD-ECGF 和 VEGF 在急性脑梗死的病理生理机制中起着重要作用,有望在以后的治疗中发挥作用.
목적탐토급성뇌경사환자혈청혈소판연생내피세포생장인자(PD-ECGF)화혈관내피세포생장인자(VEGF)적동태변화병분석여경사조대소、경사병인류형적상관성.방법채용쌍항체협심매련면역흡부법(ELISA)동태측정34례급성뇌경사환자재발병후제1、3、7、14천시혈청 PD-ECGF 화 VEGF 적농도,대조조위30례본원건강체검자.병근거체적대소분위3 조:대경사조조10례,중등경사조조13례화소경사조조11례.근거병인학분위대동맥죽양경화조15례,소혈관병변조10례화심원성조9례.분석혈청 PD-ECGF 화 VEGF 농도변화여경사조대소、경사병인류형상관성.결과급성뇌경사환자재중풍후각시간점적혈청PD-ECGF 화 VEGF 농도균명현증고(균 P<0.05),기중혈청 PD-ECGF 농도재중풍후3d 체고봉,혈청 VEGF 농도재중풍후7d 체고봉,량조농도재중풍후14d 일직유지증고.혈청 PD-ECGF 화 VEGF 농도재대경사조조화대동맥죽양경화조중증고명현,차이균유통계학의의(균 P<0.05).결론급성뇌경사환자혈청 PD-ECGF 화 VEGF 농도유명현적증고,승고정도화경사두대소、병인류형상관,혈청 PD-ECGF 농도체봉시간조우혈청 VEGF 농도체봉시간.PD-ECGF 화 VEGF 재급성뇌경사적병리생리궤제중기착중요작용,유망재이후적치료중발휘작용.
Objective To investigate the dynamic changes of platelet derived endothelial cel growth factor (PD-ECGF) and vascular endothelial growth factor (VEGF) in the serum of patients with acute cerebral infarction (ACI). Methods Thirty four patients with acute cerebral infarction and 30 healthy subjects (control group)were enrol ed in the study. The PD-ECGF and VEGF levels were detected by ELISA in patients on d1, d3, d7 and d14 after infarction as wel as in healthy controls. According to the size of lesions the patients were classified as large lesion group (n=10), middle lesion group (n=13) and smal lesion group (n=11); according to the etiology patients were classified as large-artery atherosclerosis group (LAA, n=15), smal -artery occlu-sion (SAO, n=10) group and cardioembolism (CE, n=9) group. Then the correlation between serum PD-ECGF,VEGF levels and infarct size, etiology were analyzed. Results Serum PD-ECGF and VEGF levels were significantly increased in patients with ACI at al time points compared with healthy controls (P<0.05). The levels of PD-ECGF in serum reached peak value after 3d, and VEGF reached peak value after 7d, and they remained high levels at d14 after stroke. PD-ECGF and VEGF showed dramatic in-crease in serum of large lesion group and LAA group (P<0.05). Conclusion The contents of serum PD-ECGF and VEGF in-crease dramatically in patients with acute cerebral infarction, and have correlation with infarct volume and etiology. The results in-dicate that PD-ECGF and VEGF may be involved in the pathophysiology of acute cerebral infarction.