CAJ | 학술논문

目的研究 miR-145在结直肠癌患者和健康者血浆中的表达差异,并探讨其与临床特征之间的关系.方法收集53例初治结直肠癌患者和26例健康者外周血浆,提取总 RNA,用反转录及 real-time PCR 检测 miR-145的表达情况,分析其与临床特征之间的关系,评估其对结直肠癌的诊断效能.结果 miR-145在结直肠癌患者血浆中的表达较健康者显著降低(P<0.01), miR-145的 AUC 为0.859,鉴别结直肠癌患者的敏感性86.8%、特异性88.5%,但 miR-145的表达水平与临床特征(性别、年龄、肿瘤位置、淋巴结状态、TNM 分期、局部浸润深度及 CEA 水平)之间均无相关性(均 P >0.05).结论血浆 miR-145有望作为结直肠癌的新型标记物,但可能只参与了结直肠癌发生的起始过程.
목적연구 miR-145재결직장암환자화건강자혈장중적표체차이,병탐토기여림상특정지간적관계.방법수집53례초치결직장암환자화26례건강자외주혈장,제취총 RNA,용반전록급 real-time PCR 검측 miR-145적표체정황,분석기여림상특정지간적관계,평고기대결직장암적진단효능.결과 miR-145재결직장암환자혈장중적표체교건강자현저강저(P<0.01), miR-145적 AUC 위0.859,감별결직장암환자적민감성86.8%、특이성88.5%,단 miR-145적표체수평여림상특정(성별、년령、종류위치、림파결상태、TNM 분기、국부침윤심도급 CEA 수평)지간균무상관성(균 P >0.05).결론혈장 miR-145유망작위결직장암적신형표기물,단가능지삼여료결직장암발생적기시과정.
Objective To investigate the plasma expression of miR-145 in patients with colorectal cancer and its clinical significance. Methods The plasma levels of miR-145 in 53 patients with colorectal cancer and 26 healthy subjects were mea-sured using real-time quantitative PCR. The relationship between miR-145 expression and clinical characteristics of colorectal cancer was analyzed. The diagnostic value of miR-145 for colorectal cancer was evaluated. Results The plasma miR-145 lev-els were decreased significantly in colorectal cancer patients (P<0.01). The AUC (the area under the ROC curve) of miR-145 was 0.859 with a 86.8%sensitivity and 88.5%specificity in discriminating colorectal cancer from normal subjects; however, the expression levels of miR-145 were not associated with the gender, age, tumor location, nodal status, TNM staging, T-classifica-tion and CEA levels of patients (P >0.05). Conclusion A decreased plasma miR-145 levels are detected in patients with col-orectal cancer, which suggests miR-145 might be used as a potential tumor-marker for colorectal cancer.