CAJ | 학술논문

目的探讨发酵支原体(Mf)和梨支原体(Mpi)感染小鼠胸腺细胞 Fas/FasL 表达与多器官损伤相关性.方法55只清洁级 ICR 小鼠,分实验组24只(免疫抑制 Mf、Mpi 感染组)及对照组31只(非免疫抑制 Mf 感染和 NS 组).感染组分别腹腔注射0.3ml Mf 和 Mpi 标准菌株(6×108-9/ml),NS 组注入相同量0.9%氯化钠注射液.计算各组小鼠死亡率,取其血清进行支原体再培养,免疫组化法分析小鼠胸腺细胞 Fas/FasL 的表达,电镜观察死亡小鼠心、肺、肝和肾的超微结构变化.结果实验组小鼠在感染3~5d后100%死亡(24/24),与非免疫抑制 Mf 感染对照组比较有统计学差异(P<0.01).胸腺细胞 Fas/FasL 高表达与心、肝、肺和肾等多器官损伤相一致.结论 Mf 或 Mpi 感染小鼠胸腺细胞 Fas/FasL 高表达与心、肺、肝和肾多器官损伤相关.
목적탐토발효지원체(Mf)화리지원체(Mpi)감염소서흉선세포 Fas/FasL 표체여다기관손상상관성.방법55지청길급 ICR 소서,분실험조24지(면역억제 Mf、Mpi 감염조)급대조조31지(비면역억제 Mf 감염화 NS 조).감염조분별복강주사0.3ml Mf 화 Mpi 표준균주(6×108-9/ml),NS 조주입상동량0.9%록화납주사액.계산각조소서사망솔,취기혈청진행지원체재배양,면역조화법분석소서흉선세포 Fas/FasL 적표체,전경관찰사망소서심、폐、간화신적초미결구변화.결과실험조소서재감염3~5d후100%사망(24/24),여비면역억제 Mf 감염대조조비교유통계학차이(P<0.01).흉선세포 Fas/FasL 고표체여심、간、폐화신등다기관손상상일치.결론 Mf 혹 Mpi 감염소서흉선세포 Fas/FasL 고표체여심、폐、간화신다기관손상상관.
Objective To investigate expression of Fas/FasL in thymocytes and its relation with multiple organ damage in mice lethal y infected by Mycoplasma fermentans (Mf) and Mycoplasma pirum (Mpi). Methods 55 ICR mice were randomly di-vided into experimental group (immunosuppression+Mf and Mpi infection, n=24) and control groups (non-immunosuppres-sion+Mf infection, and normal group, n=31). Mice in experiment group were injected intraperitoneal y with 0.3ml (6×108-9/ml) Mf or Mpi, the NS group was injected with 0.3ml sterile normal saline. The mortality rate was evaluated and serum samples were taken for cultivation of the mycoplasma. The expression of Fas/FasL in mice thymus was analyzed with immunohistochemistry. The ul-trastructural changes of thymus, heart, lung, liver and kidney were observed with electron microscopy. Results The mice in ex-perimental group al died (24/24) within 3-5 days after infection. The expression of Fas / FasL in thymocytes was high, which was consistent with the extent of damage in the heart, liver, lung and kidney. Conclusion The high expression of Fas/FasL in thymo-cytes is correlated with the multi-organ damage in Mf and Mpi infected mice.