CAJ | 학술논문

目的分析RhD阴性女性妊娠期的意外抗体筛选情况.方法利用盐水介质法、聚凝胺法和抗人球蛋白法对376例Rh阴性妊娠期女性进行了意外抗体筛查和抗体效价的测定,并对结果进行回顾性分析.结果33例检出意外抗体,检出率为8.8%(33/376).检出抗体为抗D 24例(72.7%),抗D+C 2例(6.1%),抗E 2例(6.1%),抗Fyb 1例(3.0%),抗M 1例(3.0%)和抗P 11例(3.0%),2例未能鉴定出抗体特性.376例Rh阴性受检者中78例筛查2次以上,其中10例第1次筛查阳性而在随后定期检测抗体滴度呈现变化,包括8例呈现上升趋势和2例下降趋势;4例保持不变;3例第1次筛查阴性而在随后的定期检测呈阳性,61例第1次筛查阴性而在随后的检测中未发现意外抗体.结论 RhD阴性妊娠期女性中存在一定比例的意外抗体,定期筛查抗体有助于指导临床及时采用干预措施.
목적분석RhD음성녀성임신기적의외항체사선정황.방법이용염수개질법、취응알법화항인구단백법대376례Rh음성임신기녀성진행료의외항체사사화항체효개적측정,병대결과진행회고성분석.결과33례검출의외항체,검출솔위8.8%(33/376).검출항체위항D 24례(72.7%),항D+C 2례(6.1%),항E 2례(6.1%),항Fyb 1례(3.0%),항M 1례(3.0%)화항P 11례(3.0%),2례미능감정출항체특성.376례Rh음성수검자중78례사사2차이상,기중10례제1차사사양성이재수후정기검측항체적도정현변화,포괄8례정현상승추세화2례하강추세;4례보지불변;3례제1차사사음성이재수후적정기검측정양성,61례제1차사사음성이재수후적검측중미발현의외항체.결론 RhD음성임신기녀성중존재일정비례적의외항체,정기사사항체유조우지도림상급시채용간예조시.
Objective To screen unexpected antibody in RhD-negative pregnant women. Methods Total 376 Rh-neg-ative pregnant women were analyzed retrospectively. The screening of unexpected antibody and detection of antibody titer were conducted by salt medium, polyamine and antiglobulin methods. Results Unexpected antibodies were found in 33 cases ac-counting for 8.8%of total (33/376), including 24 cases with anti-D (72.7%), 2 anti-D combined anti-C (6.1%), 2 anti-E (6.1%), 1 anti-Fyb(3.0%), 1 anti-P1 (3.0%)and 1anti-M (3.0%);and the antibodies specificity was not identified in 2 cases(6.1%). Seventy eight out of 376 cases were screened more than twice. During the fol ow-up among 14 positive cases 8 had increased antibodies titer, 2 had decreased titer and 4 had no change;while among 64 negative cases unexpected antibodies turned to positive in 3 cases and 61 cases remained negative. Conclusion Unexpected antibodies exist in serum of RhD-negative pregnant women, which indicates that regularly screening of unexpected antibody wil help to adopt intervention strategy promptly for the RhD-negative pregnant women.