CAJ | 학술논문

目的研究内源性大麻素代谢酶脂肪酸酰胺水解酶(FAAH)在脑胶质瘤组织中的表达情况及其与胶质瘤病理级别的关系,探讨其临床意义.方法选取首次手术切除并经病理检查证实的脑胶质瘤标本23例,其中WHOⅠ级1例,Ⅱ级7例,Ⅲ级5例,Ⅲ~Ⅳ级2例,Ⅳ级8例.另取8例因颅脑外伤行内减压术切除的正常脑组织作为对照.应用实时荧光定量PCR和高效液相色谱-质谱联用技术分别检测各组织标本中FAAH mRNA和FAAH活性的表达.结果 FAAH mRNA、FAAH活性在低级别(WHOⅠ~Ⅱ级)和高级别(WHOⅢ~Ⅳ级)脑胶质瘤组织中的表达水平较正常脑组织明显降低,差异有统计学意义(均P<0.01),且随着肿瘤病理级别的增高而降低,与肿瘤的病理分级呈负相关(均P<0.01).FAAH mRNA与FAAH活性的表达水平呈正相关(P<0.01).结论 FAAH可能参与了脑胶质瘤的发生、发展过程,检测FAAH的表达可评价脑胶质瘤的生物学行为,为脑胶质瘤的临床治疗和预后判断提供参考.
목적연구내원성대마소대사매지방산선알수해매(FAAH)재뇌효질류조직중적표체정황급기여효질류병리급별적관계,탐토기림상의의.방법선취수차수술절제병경병리검사증실적뇌효질류표본23례,기중WHOⅠ급1례,Ⅱ급7례,Ⅲ급5례,Ⅲ~Ⅳ급2례,Ⅳ급8례.령취8례인로뇌외상행내감압술절제적정상뇌조직작위대조.응용실시형광정량PCR화고효액상색보-질보련용기술분별검측각조직표본중FAAH mRNA화FAAH활성적표체.결과 FAAH mRNA、FAAH활성재저급별(WHOⅠ~Ⅱ급)화고급별(WHOⅢ~Ⅳ급)뇌효질류조직중적표체수평교정상뇌조직명현강저,차이유통계학의의(균P<0.01),차수착종류병리급별적증고이강저,여종류적병리분급정부상관(균P<0.01).FAAH mRNA여FAAH활성적표체수평정정상관(P<0.01).결론 FAAH가능삼여료뇌효질류적발생、발전과정,검측FAAH적표체가평개뇌효질류적생물학행위,위뇌효질류적림상치료화예후판단제공삼고.
Objective To investigate the expression of endocannabinoid metabolic enzyme fatty acid amide hydrolase (FAAH)in human brain glioma tissues and its clinicopathological significance. Methods Twenty-three patients with brain glioma, including one case with grade I, 7 grade II, 5 grade III,2 grade III~IV and 8 glioblastoma, undergoing surgical excision were enrol ed in the study; and 8 samples of normal brain tissues from patients undergoing decompression operation due to cerebral hernia served as controls. qRT-PCR and HPLC-MS were used to detect the mRNA and the activity of FAAH, respec-tively. Results The expression of mRNA and activity of FAAH was significantly higher in low grade (gradeⅠ~Ⅱ) and high grade (gradeⅢ~Ⅳ) brain glioma than those in the normal brain tissues (P<0.01). The expression of FAAH mRNA was positively corre-lated with the FAAH Activity,and both were negatively correlated with the pathological grades (P<0.01). Conclusion FAAH may be involved in the occurrence and development of brain glioma;the detection of FAAH expression may be of value in evaluation of biological behavior and prognosis of brain glioma.